PMID: 11328276May 1, 2001Paper

A DNA-based immunization protocol to produce monoclonal antibodies to blood group antigens

British Journal of Haematology
T H Tearina ChuMarion E Reid

Abstract

A major challenge facing transfusion medicine is the establishment of immunological methods to produce specific and avid blood group typing reagents to the many polymorphic blood group antigens. This is especially true when sources of human antibody are limited. Based on the knowledge that inoculation with plasmid DNA can induce a humoral response in the host animal, we inoculated mice with plasmid DNA followed by a single boost injection with plasmid-transfected cells that have a high level of expression of the same target protein. Using this method, several hybridoma clones that produced strongly reactive antibodies specific for the Kell polymorphic antigens (anti-K, anti-k, anti-Kp(a)) were isolated. The monoclonal antibodies that were produced with this method have potential clinical utility for identifying a patient's blood type and for screening for antigen-negative donor blood.

References

Jul 31, 1992·Journal of Immunological Methods·P G SteenbakkersW Olijve
Mar 23, 1990·Science·J A WolffP L Felgner
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·M L MichelR G Whalen
Dec 1, 1994·Vaccine·D Xu, F Y Liew
Mar 19, 1993·Science·J Cohen
Aug 1, 1996·Trends in Microbiology·D E Hassett, J L Whitton
Jan 1, 1997·Annual Review of Immunology·J J DonnellyM A Liu
Jan 1, 1997·Vox Sanguinis·S Lee
Feb 19, 2000·American Journal of Hematology·K YazdanbakhshM E Reid
Mar 1, 1957·Vox Sanguinis·F H ALLEN, S J LEWIS

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Citations

Apr 2, 2003·Journal of Virological Methods·Chunya PuttikhuntPrida Malasit
Jun 15, 2005·ILAR Journal·Michael K Schunk, G Eileen Macallum
Feb 15, 2002·Current Opinion in Hematology·Marion E Reid, Christine Lomas-Francis
Jan 25, 2011·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Marion E Reid, Gregory A Denomme
Apr 15, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J D A RamosK Y Chua

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