A DNA-directed DNA polymerase from murine liver mitochondria.

Biochimica Et Biophysica Acta
N B Hecht

Abstract

A DNA-directed DNA polymerase has been isolated from murine liver mitochondria. The mitochondrial DNA polymerase is distinguishable from other DNA polymerases found in the nucleus and cytosol of murine cells by several enzymatic and physical properties. It is stimulated 5--6-fold by 0.15 M KCl, does not require a sulfhydryl reducing agent for activity, and is inhibited by ethidium bromide or ATP. The enzyme has a sedimentation coefficient of 8.8 S in the presence of up to 0.5 M KCl, a molecular weight of 150--170000, and utilizes natural templates in the following order of preference: activated DNA (100%), single stranded DNA (24%), and native DNA (5%).

References

Oct 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R G Smith, R C Gallo
Dec 1, 1974·Journal of Reproduction and Fertility·N B Hecht
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A MatsukageS H Wilson
Jan 4, 1973·Biochemical and Biophysical Research Communications·G S Probst, R R Meyer
Feb 15, 1973·Journal of Molecular Biology·L M ChangF J Bollum
Sep 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R R Meyer, M V Simpson
Oct 5, 1966·Biochemical and Biophysical Research Communications·E Wintersberger

❮ Previous
Next ❯

Citations

Mar 1, 1984·Biochimie·A C DockR Giégé
Aug 1, 1978·Nucleic Acids Research·W JoesterP H Hofschneider
Aug 23, 1976·Biochemical and Biophysical Research Communications·K Radsak, G Seidel
Dec 11, 1976·Archives of Biochemistry and Biophysics·L J Klunk, G F Kalf
Aug 1, 1976·Hoppe-Seyler's Zeitschrift für physiologische Chemie·K Radsak, A Weissbach
Aug 15, 1978·European Journal of Biochemistry·K Radsak, E Schütz
Dec 1, 1977·European Journal of Biochemistry·U HübscherS Spadari
Apr 19, 1977·Biochimica Et Biophysica Acta·T FujisawaK Koike
Dec 2, 1977·Biochimica Et Biophysica Acta·S Tanaka, K Koike
Dec 22, 1983·Biochimica Et Biophysica Acta·G StauderK E Joester

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
F RougeonF Chapeville
Biochemical and Biophysical Research Communications
B S Vold
© 2022 Meta ULC. All rights reserved