A DNA duplex with extremely enhanced thermal stability based on controlled immobilization on gold nanoparticles

Nano Letters
Kensuke AkamatsuNaoki Sugimoto

Abstract

The effect of DNA loadings on the thermal stability of DNA duplex immobilized on gold nanoparticles has been investigated. The modestly loaded duplexes on the gold nanoparticles showed enhanced thermal stability, as compared to that of the free duplex (without gold nanoparticles). However, the highly loaded duplex showed stability similar to that of free duplex. The stability could be controlled over a wide temperature range simply by varying the salt concentration (over 50 degrees C). Additionally, the gold nanoparticles with modestly loaded oligonucleotides could be used as nanoprobes for effective and fast strand exchange reactions, based on the increased thermal stability of the immobilized duplex. These results indicate that the interaction between the duplex and the nanoparticle surface plays an important role in determining the stability of the duplex.

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Citations

Jun 12, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Evan S KrystofiakJulie A Oliver
Feb 26, 2010·Nucleosides, Nucleotides & Nucleic Acids·Guofan WuJingwan Kang
Apr 22, 2009·Nucleic Acids Research·Chunlai ChenXin Sheng Zhao
Jan 29, 2014·Current Opinion in Biotechnology·Seyed-Fakhreddin Torabi, Yi Lu
Sep 23, 2014·Bioconjugate Chemistry·Xiaoye SuJanarthanan Jayawickramarajah
Mar 12, 2008·Biochimie·Daisuke Miyoshi, Naoki Sugimoto
Jul 22, 2011·Analytical Chemistry·Shu-ichi NakanoNaoki Sugimoto
Sep 27, 2008·Small·Won-Kyu RhimJwa-Min Nam
Dec 14, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Naoki KanayamaMizuo Maeda
Feb 25, 2017·Biomacromolecules·Ngozi A EzeValeria T Milam
May 24, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Wenting ZhaoI-Ming Hsing

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