A DNA-gold nanoparticle-based colorimetric competition assay for the detection of cysteine

Nano Letters
Jae-Seung LeeChad A Mirkin

Abstract

We report the development of a highly sensitive and selective colorimetric detection method for cysteine based upon oligonucleotide-functionalized gold nanoparticle probes that contain strategically placed thymidine-thymidine (T-T) mismatches complexed with Hg2+. This assay relies upon the distance-dependent optical properties of gold nanoparticles, the sharp melting transition of oligonucleotide-linked nanoparticle aggregates, and the very selective coordination of Hg2+ with cysteine. The concentration of cysteine can be determined by monitoring with the naked eye or a UV-vis spectrometer the temperature at which the purple-to-red color change associated with aggregate dissociation takes place. This assay does not utilize organic cosolvents, enzymatic reactions, light-sensitive dye molecules, lengthy protocols, or sophisticated instrumentation thereby overcoming some of the limitations of more conventional methods.

References

Aug 15, 1996·Nature·A P AlivisatosP G Schultz
Jul 1, 1996·British Journal of Urology·N SaravananP Varalakshmi
Dec 24, 1995·Brain Research·M Puka-SundvallA Lehmann
Nov 21, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·W Dröge, E Holm
Nov 4, 2000·Neurochemical Research·R JanákyS S Oja
Aug 9, 2002·Journal of the American Chemical Society·Dustin J MaxwellShuming Nie
Jan 15, 2004·Journal of the American Chemical Society·Oleksandr RusinRobert M Strongin
Sep 24, 2004·Journal of the American Chemical Society·Valeri PavlovItamar Willner
May 5, 2005·Journal of the American Chemical Society·P K SudeepK George Thomas
Dec 15, 2005·Analytical Chemistry·C Shad ThaxtonChad A Mirkin
Jan 5, 2006·Inorganic Chemistry·Farideh JalilehvandEmiliana Damian
Jan 13, 2006·Nano Letters·Lisa M DillenbackChristine D Keating
Feb 16, 2006·Journal of the American Chemical Society·Yoko MiyakeAkira Ono
Apr 8, 2006·Angewandte Chemie·Savka I StoevaChad A Mirkin
May 13, 2006·Analytical Chemistry·Marta G CerrutiStefan Franzen
Jun 29, 2006·Journal of the American Chemical Society·Savka I StoevaChad A Mirkin
Jan 11, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kouki OkuboNobuo Uehara

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Citations

Feb 4, 2009·Analytical and Bioanalytical Chemistry·Marc R Knecht, Manish Sethi
Jul 31, 2009·Analytical and Bioanalytical Chemistry·Zhang ChenQingyun Cai
Mar 25, 2010·Analytical and Bioanalytical Chemistry·Tong WuCheng Zhi Huang
Jan 31, 2012·Analytical and Bioanalytical Chemistry·Chia-Chen ChangChii-Wann Lin
Feb 22, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xin Yan HouYun Fei Long
Jul 27, 2011·ACS Applied Materials & Interfaces·Jia ZhangXiurong Yang
Feb 3, 2012·Chemical Reviews·Krishnendu SahaVincent M Rotello
Jan 31, 2013·Molecular Pharmaceutics·Aneta J MieszawskaDavid P Cormode
Dec 1, 2012·Nano Letters·Xiaoqing LiuItamar Willner
Apr 14, 2010·Chemical Communications : Chem Comm·Jiaofang LiuLinyong Zhu
Jun 5, 2010·Chemical Communications : Chem Comm·Yi-Bin RuanYun-Bao Jiang
Mar 10, 2011·Chemical Communications : Chem Comm·Yuling Wang, Joseph Irudayaraj
Jul 13, 2011·Chemical Communications : Chem Comm·Xi ZhuGuonan Chen
Dec 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·Zhen-Gang WangItamar Willner
Apr 14, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Fei YangZhiyong Guo
Jun 10, 2009·Nanomedicine·Melissa M KempRobert J Linhardt
May 31, 2014·Annals of Biomedical Engineering·Ricky Y T ChiuDaniel T Kamei
Jun 24, 2014·The Journal of Chemical Physics·Xiankai JiangBo Song
Mar 12, 2013·Journal of the American Chemical Society·Stanley S ChouVinayak P Dravid
May 21, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sungsook AhnSang Joon Lee
Sep 12, 2009·Expert Opinion on Drug Delivery·Satish K NuneCory J Berkland
Jan 21, 2016·Analytical Chemistry·Mengyuan HeZhihong Liu
Dec 10, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Isja de FeijterIlja K Voets

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