PMID: 9186955May 1, 1997Paper

A DNA hybridization method for typing hepatitis C virus genotype 2c

Journal of Virological Methods
M R BiasinD Infantolino

Abstract

A high prevalence of hepatitis C virus (HCV) genotype 2c (22%) was detected in sera from 459 italian patients by core-region amplification and hybridization with specific probes by DNA enzyme immunoassay. Amplified fragments failed to hybridize with 1a, 1b, 2a, 2b and 3a subtype-specific and 4, 5, 6 type-specific oligonucleotides in 105 patients. Hybridization of these samples with type 2 probe, which recognized all the subtypes sequences, showed evidence for genotype 2 distinct from 2a and 2b. Fourteen out of these 105 isolates were cloned and sequenced. The results were consistent with genotyping assay. Nucleotide sequences were partially related to types 2a, 2b, 2d, 2e and 2f (87.0-93.5% of identity). The average nucleotide identity was highest for genotype 2c (95.87%). On the basis of sequence analysis, subtype 2c specific probe was derived. Hybridization efficiency with the newly designed probe was very high and more than 95% (100/105) of type 2 cases were classified as 2c. Evidence of different outcome of therapy inside the same HCV major type account for the need of accurate subtyping. In this study, amplification of the core region followed by hybridization with highly specific probes enabled distinction between HCV sub...Continue Reading

References

Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J BukhR H Miller
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J BukhR H Miller
Oct 1, 1991·Journal of Virological Methods·L ImbertiD Primi
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Q L ChooP J Barr
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·N OgataR H Purcell
Nov 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Y KanazawaT Kamada
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·L StuyverG Maertens
Feb 1, 1995·Seminars in Liver Disease·J BukhR H Purcell
May 1, 1994·Digestive Diseases and Sciences·E MitaT Kamada
Nov 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M HondaK Kobayashi
Jun 1, 1994·Journal of Virological Methods·S ViazovM Roggendorf
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·H TokitaM Mayumi
Jul 1, 1994·Antiviral Research·C TrépoL Vitvitski
Jul 1, 1994·The Journal of General Virology·M SakamotoH Okamoto
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·J BukhR H Miller
May 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P SimmondsD S Chen
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J BukhR H Miller

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Citations

Jan 6, 2000·Journal of Hepatology·J M Pawlotsky
Oct 23, 1998·Research in Virology·E SpadaM Rapicetta
Nov 28, 2012·BMC Infectious Diseases·Massimo CiccozziMarco Ciotti
Oct 9, 1999·Clinical Microbiology Reviews·M Arens

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