A DNA repair gene of Caenorhabditis elegans: a homolog of human XPF

DNA Repair
Hye Kyung ParkByungchan Ahn

Abstract

The xeroderma pigmentosum complementation group F (XPF) protein is a structure-specific endonuclease in a complex with ERCC1 and is essential for nucleotide excision repair (NER). We report a single cDNA of Caenorhabditis elegans (C. elegans) encoding highly similar protein to human XPF and other XPF members. We propose to name the corresponding C. elegans gene xpf. Messenger RNA for C. elegans xpf is 5'-tagged with a SL2 splice leader, suggesting an operon-like expression for xpf. Using RNAi, we showed that loss of C. elegans xpf function caused hypersensitivity to ultra-violet (UV) irradiation, as observed in enhanced germ cell apoptosis and increased embryonic lethality. This study suggests that C. elegans xpf is conserved in evolution and plays a role in the repair of UV-damaged DNA in C. elegans.

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Citations

Sep 30, 2014·Journal of Agricultural and Food Chemistry·Alberto PalmaJose M Rodríguez Mellado
Jan 22, 2008·Nucleic Acids Research·Moonjung HyunByungchan Ahn
Jun 21, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Maxwell C K LeungJoel N Meyer
Oct 20, 2006·BMC Genomics·Erin J GilchristGeorge W Haughn
May 3, 2007·Genome Biology·Joel N MeyerBennett Van Houten
Nov 18, 2011·Molecular Biology International·Hannes Lans, Wim Vermeulen
Nov 22, 2018·Animal Cells and Systems·Bala Murali Krishna VasamsettiNam Jeong Cho

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