A DNA vaccine against tuberculosis based on the 65 kDa heat-shock protein differentially activates human macrophages and dendritic cells.

Genetic Vaccines and Therapy
Luís H FrancoVânia L D Bonato

Abstract

A number of reports have demonstrated that rodents immunized with DNA vaccines can produce antibodies and cellular immune responses presenting a long-lasting protective immunity. These findings have attracted considerable interest in the field of DNA vaccination. We have previously described the prophylactic and therapeutic effects of a DNA vaccine encoding the Mycobacterium leprae 65 kDa heat shock protein (DNA-HSP65) in a murine model of tuberculosis. As DNA vaccines are often less effective in humans, we aimed to find out how the DNA-HSP65 stimulates human immune responses. To address this question, we analysed the activation of both human macrophages and dendritic cells (DCs) cultured with DNA-HSP65. Then, these cells stimulated with the DNA vaccine were evaluated regarding the expression of surface markers, cytokine production and microbicidal activity. It was observed that DCs and macrophages presented different ability to uptake DNA vaccine. Under DNA stimulation, macrophages, characterized as CD11b+/CD86+/HLA-DR+, produced high levels of TNF-alpha, IL-6 (pro-inflammatory cytokines), and IL-10 (anti-inflammatory cytokine). Besides, they also presented a microbicidal activity higher than that observed in DCs after infecti...Continue Reading

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Citations

Feb 7, 2009·Expert Review of Vaccines·Celio L SilvaAlexandrina Sartori
Jun 15, 2011·Vaccine·Louise von Gersdorff Jørgensen, Kurt Buchmann
Sep 6, 2008·Developmental and Comparative Immunology·Kenneth C McCullough, Artur Summerfield
May 12, 2016·BMC Biotechnology·Gecilmara Salviato PileggiCelio L Silva
Jan 7, 2017·Human Vaccines & Immunotherapeutics·Pryscilla Fanini WowkVânia Luiza Deperon Bonato
Oct 8, 2020·Metabolites·Haoxin ZhaoStanley Ching-Cheng Huang

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
transfection
density gradient centrifugation
flow cytometry
ELISA
PCR
electrophoresis
confocal microscopy
fluorescence microscopy

Software Mentioned

Quest
PRISM
GraphPad
Cell

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