A DNA vaccine induces SARS coronavirus neutralization and protective immunity in mice

Zhi-Yong YangGary J Nabel


Public health measures have successfully identified and contained outbreaks of the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), but concerns remain over the possibility of future recurrences. Finding a vaccine for this virus therefore remains a high priority. Here, we show that a DNA vaccine encoding the spike (S) glycoprotein of the SARS-CoV induces T cell and neutralizing antibody responses, as well as protective immunity, in a mouse model. Alternative forms of S were analysed by DNA immunization. These expression vectors induced robust immune responses mediated by CD4 and CD8 cells, as well as significant antibody titres, measured by enzyme-linked immunosorbent assay. Moreover, antibody responses in mice vaccinated with an expression vector encoding a form of S that includes its transmembrane domain elicited neutralizing antibodies. Viral replication was reduced by more than six orders of magnitude in the lungs of mice vaccinated with these S plasmid DNA expression vectors, and protection was mediated by a humoral but not a T-cell-dependent immune mechanism. Gene-based vaccination for the SARS-CoV elicits effective immune responses that generate protective immunity in an animal model.


Nov 1, 1993·Veterinary Microbiology·L J Saif
Jun 3, 2000·Annual Review of Immunology·S GurunathanR A Seder
Aug 16, 2001·Archives of Virology·H D LehmkuhlL W Woods
Apr 3, 2003·The New England Journal of Medicine·Susan M PoutanenUNKNOWN Canadian Severe Acute Respiratory Syndrome Study Team
Apr 3, 2003·The New England Journal of Medicine·Kenneth W TsangKar N Lai
Apr 16, 2003·The New England Journal of Medicine·Nelson LeeJoseph J Y Sung
Apr 12, 2003·The New England Journal of Medicine·Christian DrostenHans Wilhelm Doerr
Apr 12, 2003·The New England Journal of Medicine·Thomas G KsiazekUNKNOWN SARS Working Group
May 6, 2003·Science·Marco A MarraRachel L Roper
May 16, 2003·Nature·Ron A M FouchierAlbert D M E Osterhaus
Oct 31, 2003·Nature·Byron E E MartinaAlbert D M E Osterhaus
Nov 12, 2003·Journal of Virology·Wing-Pui KongGary J Nabel

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Oct 13, 2006·Human Gene Therapy·Margaret A LiuGunilla B Karlsson Hedestam
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enzyme-linked immunosorbent assay
flow cytometry

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