A DNA vaccine induces SARS coronavirus neutralization and protective immunity in mice

Nature
Zhi-Yong YangGary J Nabel

Abstract

Public health measures have successfully identified and contained outbreaks of the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), but concerns remain over the possibility of future recurrences. Finding a vaccine for this virus therefore remains a high priority. Here, we show that a DNA vaccine encoding the spike (S) glycoprotein of the SARS-CoV induces T cell and neutralizing antibody responses, as well as protective immunity, in a mouse model. Alternative forms of S were analysed by DNA immunization. These expression vectors induced robust immune responses mediated by CD4 and CD8 cells, as well as significant antibody titres, measured by enzyme-linked immunosorbent assay. Moreover, antibody responses in mice vaccinated with an expression vector encoding a form of S that includes its transmembrane domain elicited neutralizing antibodies. Viral replication was reduced by more than six orders of magnitude in the lungs of mice vaccinated with these S plasmid DNA expression vectors, and protection was mediated by a humoral but not a T-cell-dependent immune mechanism. Gene-based vaccination for the SARS-CoV elicits effective immune responses that generate protective immunity in an animal model.

References

Nov 1, 1993·Veterinary Microbiology·L J Saif
Jun 3, 2000·Annual Review of Immunology·S GurunathanR A Seder
Aug 16, 2001·Archives of Virology·H D LehmkuhlL W Woods
Apr 3, 2003·The New England Journal of Medicine·Susan M PoutanenUNKNOWN Canadian Severe Acute Respiratory Syndrome Study Team
Apr 3, 2003·The New England Journal of Medicine·Kenneth W TsangKar N Lai
Apr 16, 2003·The New England Journal of Medicine·Nelson LeeJoseph J Y Sung
Apr 12, 2003·The New England Journal of Medicine·Christian DrostenHans Wilhelm Doerr
Apr 12, 2003·The New England Journal of Medicine·Thomas G KsiazekUNKNOWN SARS Working Group
May 6, 2003·Science·Marco A MarraRachel L Roper
May 16, 2003·Nature·Ron A M FouchierAlbert D M E Osterhaus
Oct 31, 2003·Nature·Byron E E MartinaAlbert D M E Osterhaus
Nov 12, 2003·Journal of Virology·Wing-Pui KongGary J Nabel

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Citations

Sep 22, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Keiji KubaJosef M Penninger
Dec 11, 2008·Immunologic Research·Shaheed A Abdulhaqq, David B Weiner
Oct 21, 2010·Virologica Sinica·Yu-xuan HouZheng-li Shi
Jun 14, 2005·Current Opinion in Immunology·Andreas Wack, Rino Rappuoli
Mar 8, 2005·Drug Discovery Today·Yossef KligerDoron Gerber
Mar 16, 2005·The Lancet Infectious Diseases·David A GronebergPeter Zabel
Dec 4, 2004·Nature Medicine·J S M PeirisK Y Yuen
Feb 10, 2009·Nature Reviews. Microbiology·Lanying DuShibo Jiang
Jun 16, 2004·Nature Reviews. Microbiology·B Brett FinlayRobert C Brunham
Sep 15, 2007·The New England Journal of Medicine·Wu-Chun CaoJan H Richardus
Aug 20, 2004·The New England Journal of Medicine·Robert E Johnston
Aug 5, 2009·The Journal of Experimental Medicine·Sarah M McWhirterRussell E Vance
Jun 15, 2007·Antioxidants & Redox Signaling·Emmanuel FenouilletIan M Jones
Aug 2, 2007·DNA and Cell Biology·Alexander N ZakhartchoukLorne A Babiuk
Oct 13, 2006·Human Gene Therapy·Margaret A LiuGunilla B Karlsson Hedestam
Jul 23, 2005·Viral Immunology·Yuxian He, Shibo Jiang
Apr 1, 2005·Nucleic Acids Research·Brock F BinkowskiPeter J Belshaw
Feb 1, 2007·The Nurse Practitioner·Anita HunterLorna Schumann
Dec 4, 2004·The Pediatric Infectious Disease Journal·Mark R Denison
Aug 17, 2012·Science Translational Medicine·Chih-Jen WeiGary J Nabel
Oct 17, 2006·Clinical Microbiology Reviews·Laura Gillim-Ross, Kanta Subbarao
Oct 16, 2007·Clinical Microbiology Reviews·Vincent C C ChengKwok Yung Yuen
Apr 21, 2012·Clinical and Vaccine Immunology : CVI·Keyan ChenFeng Gao

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Datasets Mentioned

BETA
AY278741

Methods Mentioned

BETA
transfection
enzyme-linked immunosorbent assay
ELISA
flow cytometry
Assay

Software Mentioned

FlowJo

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