A domain that assumes a Z-conformation includes a specific deletion in some cloned variants of a complex satellite

Gene
R F FowlerD M Skinner

Abstract

Sequence analyses show that deletions of 10 and 12 bp occur at homologous sites in a domain that is rich in alternating purines and pyrimidines (Pu/Py) in B42 and EXT, two cloned variants of a complex satellite DNA. A 3-bp deletion occurs 27 bp upstream from the site of the specific deletions in B42 and RU, a third cloned satellite variant that has not suffered the 10-bp deletion. Under torsional stress, the Pu/Py-rich domain adopts a Z-conformation as shown by (i) inhibition of cutting at a BssHII site that accounts for 2/5 of a 15-bp tract of pure Pu/Py in the domain; (ii) binding of polyclonal and monoclonal anti-Z-DNA antibodies to the domain; and (iii) antibody stabilization and subsequent relaxation of the Z-region.

References

Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·W Keller
Jan 1, 1978·Annual Review of Biophysics and Bioengineering·W R Bauer
Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D E PulleyblankH P Vosberg
Mar 11, 1985·Nucleic Acids Research·F S Lee, W R Bauer
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A H WangA Rich
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J K Barton, A L Raphael
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·F AzorinA Rich
Jan 1, 1983·Human Genetics·D N Cooper
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D M Lilley
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C K SingletonR D Wells
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·D M SkinnerR F Fowler
Jan 1, 1984·Annual Review of Biochemistry·A RichA H Wang
Oct 1, 1983·Journal of Biomolecular Structure & Dynamics·T M JovinF Eckstein
Sep 6, 1982·FEBS Letters·S Urieli-ShovalA Razin
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E M LaferA Rich
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L J PeckJ C Wang
Jul 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·D M Skinner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.