A dot immunobinding assay on nitrocellulose for the detection of bovid herpesvirus-4 antibodies

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
K Naeem, S M Goyal

Abstract

A dot immunobinding assay (DIA) was developed for the detection of antibody against bovid herpesvirus-4 (BHV-4) in bovine sera. A semipurified virus preparation was used as the antigen and an antispecies horseradish peroxidase-labeled IgG and diaminobenzidine were employed as the detection system. The sensitivity and specificity of the DIA were similar to those of indirect fluorescent antibody test, indicating the suitability of DIA as a rapid field test for the detection of anti-BHV-4 antibodies in cattle.

Citations

Dec 18, 2012·Archives of Virology·Manu AsthanaRajesh Kumar
Aug 4, 2006·Veterinary Research Communications·V Balamurugan, J M Kataria
Feb 28, 2007·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Megan W BlasierRobyn Barbiers
Jun 3, 1999·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·M EssmailA A Hegazy

Related Concepts

Metazoa
Antibodies, Viral
Bos indicus
Cattle Diseases
Enzyme-Linked Immunosorbent Assay
Immunofluorescence Assay
Thymic Group Viruses
Herpesviridae Infections
Immune Sera

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