A double-network poly(Nɛ-acryloyl L-lysine)/hyaluronic acid hydrogel as a mimic of the breast tumor microenvironment

Acta Biomaterialia
Weijun XuHejing Wang

Abstract

To mimic the structure of breast tumor microenvironment, novel double-network poly(Nɛ-acryloyl L-lysine)/hyaluronic acid (pLysAAm/HA) hydrogels were fabricated by a two-step photo-polymerization process for in vitro three-dimensional (3D) cell culture. The morphology, mechanical properties, swelling and degradation behaviors of pLysAAm/HA hydrogels were investigated. The growth behavior and function of MCF-7 cells cultured on the hydrogels and standard 2D culture plates were compared. The results showed that pLysAAm/HA hydrogels had a highly porous microstructure with a double network and that their mechanical properties, swelling ratio and degradation rate depended on the degree of methacrylation of HA. The results of in vitro studies revealed that the pLysAAm/HA hydrogels could support MCF-7 cell adhesion, promote cell proliferation, and induce the diversification of cell morphologies and overexpression of VEGF, IL-8 and bFGF. The MCF-7 cells cultured on 3D hydrogels showed significantly increased migration and invasion abilities as compared to 2D-cultured cells. Preliminary in vivo results confirmed that the 3D culture of MCF-7 cells resulted in greater tumorigenesis than their 2D culture. These results indicate that the pLy...Continue Reading

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Citations

Apr 5, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Benjamin TeongMei-Ling Ho
Oct 30, 2016·International Journal of Biological Macromolecules·Abid HussainMohammad Zuber
Jan 20, 2018·Advanced Healthcare Materials·Darren RodenhizerAlison P McGuigan
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May 15, 2018·ACS Biomaterials Science & Engineering·Anna M SitarskiMichaela R Reagan

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