A downy mildew effector attenuates salicylic acid-triggered immunity in Arabidopsis by interacting with the host mediator complex

PLoS Biology
Marie-Cecile CaillaudJonathan D G Jones

Abstract

Plants are continually exposed to pathogen attack but usually remain healthy because they can activate defences upon perception of microbes. However, pathogens have evolved to overcome plant immunity by delivering effectors into the plant cell to attenuate defence, resulting in disease. Recent studies suggest that some effectors may manipulate host transcription, but the specific mechanisms by which such effectors promote susceptibility remain unclear. We study the oomycete downy mildew pathogen of Arabidopsis, Hyaloperonospora arabidopsidis (Hpa), and show here that the nuclear-localized effector HaRxL44 interacts with Mediator subunit 19a (MED19a), resulting in the degradation of MED19a in a proteasome-dependent manner. The Mediator complex of ∼25 proteins is broadly conserved in eukaryotes and mediates the interaction between transcriptional regulators and RNA polymerase II. We found MED19a to be a positive regulator of immunity against Hpa. Expression profiling experiments reveal transcriptional changes resembling jasmonic acid/ethylene (JA/ET) signalling in the presence of HaRxL44, and also 3 d after infection with Hpa. Elevated JA/ET signalling is associated with a decrease in salicylic acid (SA)-triggered immunity (SATI)...Continue Reading

References

Feb 1, 1982·Molecular and Cellular Biology·H Okayama, P Berg
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·W Tao, A J Levine
Jul 19, 2001·Nature·J L Dangl, J D Jones
Sep 27, 2001·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Y SasakiS Tabata
Jan 16, 2002·Current Biology : CB·Jens S AndersenAngus I Lamond
May 2, 2002·Genome Biology·Peter KosarevChristian S Hardtke
Feb 1, 2003·Life Sciences·Yoko HoriuchiKoichiro Kawashima
Mar 4, 2003·Nature Reviews. Molecular Cell Biology·Masafumi Muratani, William P Tansey
Jun 20, 2003·Nature·Pablo D Cerdán, Joanne Chory
Aug 13, 2004·Molecular Plant-microbe Interactions : MPMI·Tom TytgatGodelieve Gheysen
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Oct 27, 2004·The Plant Journal : for Cell and Molecular Biology·Paola VeroneseTesfaye Mengiste
Dec 21, 2004·Nucleic Acids Research·John W S BrownDavid F Marshall
Jan 7, 2005·Nature·Jens S AndersenMatthias Mann
Jul 4, 2006·The Plant Journal : for Cell and Molecular Biology·Peter BrodersenJohn Mundy
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
Dec 29, 2006·The Journal of Biological Chemistry·Shamara M BaidoobonsoLawrence C Myers
Feb 14, 2007·Plant Cell Reports·Choonkyun JungJong-Joo Cheong
Jun 15, 2007·Molecular Cell·Stefan BäckströmStefan Björklund
Jan 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Maike C RentelBrian J Staskawicz
Jul 11, 2008·Plant Physiology·Heather KnightHarriet G McWatters
Jul 29, 2008·Current Opinion in Plant Biology·Anna BlockJames R Alfano
Oct 15, 2008·Molecular Cell·Maria Divina E DeatoRobert Tjian
Dec 11, 2008·The Plant Journal : for Cell and Molecular Biology·Heather KnightMarc R Knight
Jan 22, 2009·The Plant Journal : for Cell and Molecular Biology·Francesca L StefanatoHenk-jan Schoonbeek
Mar 3, 2009·Current Biology : CB·Selena Gimenez-IbanezJohn P Rathjen
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg

❮ Previous
Next ❯

Citations

Jun 29, 2014·Journal of Chemical Ecology·Marcelo L CamposGregg A Howe
May 3, 2016·Plants·Selena Gimenez-IbanezRoberto Solano
Jan 30, 2016·Nature Communications·Petra C BoevinkPaul R J Birch
Dec 17, 2014·Virulence·Prateek ChaudhariHugo Germain
Jul 15, 2015·Journal of Integrative Plant Biology·Yan YangLi-Jia Qu
Dec 15, 2015·Journal of Experimental Botany·Pin-Yao HuangLaurent Zimmerli
Dec 24, 2015·Frontiers in Plant Science·Cécile LorrainSébastien Duplessis
Sep 8, 2015·Current Opinion in Plant Biology·Shuta Asai, Ken Shirasu
Dec 30, 2015·Molecular Plant-microbe Interactions : MPMI·Oliver X DongHugo Germain
Mar 20, 2016·Journal of Experimental Botany·Irene SerranoSusana Rivas
Oct 6, 2015·Annual Review of Cell and Developmental Biology·Ruth Le FevreSebastian Schornack
Dec 30, 2014·The New Phytologist·Gunther DoehlemannJane E Parker
Jun 13, 2014·Current Opinion in Plant Biology·Edouard EvangelistiSebastian Schornack
Apr 8, 2015·Briefings in Functional Genomics·Graham B MotionEdgar Huitema
Feb 5, 2015·Molecular Plant-microbe Interactions : MPMI·Benjamin PetreSophien Kamoun
Jun 5, 2015·Frontiers in Plant Science·Shigeyuki TanakaRegine Kahmann
Apr 30, 2015·Annual Review of Plant Biology·Libera Lo PrestiRegine Kahmann
Apr 11, 2015·Frontiers in Plant Science·Lotte CaarlsSaskia C M Van Wees
Sep 10, 2014·Frontiers in Plant Science·Akira MineKenichi Tsuda
Nov 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Stan OomeGuido Van den Ackerveken
Jul 1, 2016·Annual Review of Phytopathology·Tania Y ToruñoGitta Coaker
May 14, 2016·Cell Host & Microbe·Bo LiPing He
May 20, 2017·Critical Reviews in Biochemistry and Molecular Biology·Naveen MalikAkhilesh Tyagi
May 2, 2014·Current Opinion in Plant Biology·Bilal Okmen, Gunther Doehlemann
Dec 22, 2017·The Plant Journal : for Cell and Molecular Biology·Jonathan M Plett, Francis M Martin
Jun 5, 2015·Microbiology and Molecular Biology Reviews : MMBR·Stuart FawkeSebastian Schornack
Jun 16, 2017·PLoS Pathogens·Rajesh N Patkar, Naweed I Naqvi

❮ Previous
Next ❯

Methods Mentioned

BETA
2-hybrid
transgenic
Y2H
Co-immunoprecipitation
pull-down
confocal microscopy
Fluorescence
Co-IP
ubiquitination
PCR

Software Mentioned

Primer3
Cytoscape
edgeR
Bioconductor
R
NoD
Bowtie
FASTX Toolkit

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.