A Draft Map of the Human Ovarian Proteome for Tissue Engineering and Clinical Applications.

Molecular & Cellular Proteomics : MCP
Emna OuniChristiani A Amorim

Abstract

Fertility preservation research in women today is increasingly taking advantage of bioengineering techniques to develop new biomimetic materials and solutions to safeguard ovarian cell function and microenvironment in vitro, and in vivo,. However, available data on the human ovary are limited and fundamental differences between animal models and humans are hampering researchers in their quest for more extensive knowledge of human ovarian physiology and key reproductive proteins that need to be preserved. We therefore turned to multi-dimensional label-free mass spectrometry to analyze human ovarian cortex, as it is a high-throughput and conclusive technique providing information on the proteomic composition of complex tissues like the ovary. In-depth proteomic profiling through two-dimensional liquid chromatography-mass spectrometry, Western blotting, histological and immunohistochemical analyses, and data mining helped us to confidently identify 1508 proteins. Moreover, our method allowed us to chart the most complete representation so far of the ovarian matrisome, defined as the ensemble of extracellular matrix proteins and associated factors, including more than 80 proteins. In conclusion, this study will provide a better und...Continue Reading

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Citations

Aug 31, 2019·International Journal of Molecular Sciences·Emna OuniChristiani A Amorim
Apr 7, 2020·Nature Biomedical Engineering·Emma S GargusTeresa K Woodruff
Mar 20, 2019·Molecular & Cellular Proteomics : MCP·Timothy L Karr
Dec 11, 2019·Scientific Reports·Li-Yuan Yu-LeeSue-Hwa Lin
Jul 1, 2020·Tissue Engineering. Part a·Natalie QuanFrancesca E Duncan
Sep 29, 2015·Genes & Cancer·Chitra Thakur, Fei Chen
Dec 4, 2020·International Journal of Molecular Sciences·Emna OuniChristiani A Amorim
Mar 15, 2019·Molecular & Cellular Proteomics : MCP·Timothy L Karr

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Datasets Mentioned

BETA
PXD008183

Methods Mentioned

BETA
electrophoresis
biopsy
biopsies

Software Mentioned

WebGestalt
Proteome Discoverer
Sequest HT
JMP
Leica Application Suite

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The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

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