A droplet-chip/mass spectrometry approach to study organic synthesis at nanoliter scale

Lab on a Chip
R J BeuligD Belder

Abstract

A droplet-based microfluidic device with seamless hyphenation to electrospray mass spectrometry was developed to rapidly investigate organic reactions in segmented flow providing a versatile tool for drug development. A chip-MS interface with an integrated counterelectrode allowed for a flexible positioning of the chip-emitter in front of the MS orifice as well as an independent adjustment of the electrospray potentials. This was necessary to avoid contamination of the mass spectrometer as well as sample overloading due to the high analyte concentrations. The device was exemplarily applied to study the scope of an amino-catalyzed domino reaction with low picomole amount of catalyst in individual nanoliter sized droplets.

References

Apr 19, 1996·The Journal of Organic Chemistry·Barry B. Snider, Qing Lu
Apr 22, 2004·Lab on a Chip·Miryam Fernandez-SuarezBrian H Warrington
Jan 15, 2005·Analytical Chemistry·Aaron R WheelerRobin L Garrell
May 14, 2005·Angewandte Chemie·Detlev Belder
Feb 3, 2006·Nature·Bert M Weckhuysen
Feb 24, 2006·Journal of the American Chemical Society·Takuji HatakeyamaRustem F Ismagilov
Jul 28, 2006·Nature·Jamil El-AliKlavs F Jensen
Nov 7, 2006·Angewandte Chemie·Helen SongRustem F Ismagilov
Nov 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Delai ChenRustem F Ismagilov
Nov 28, 2008·Electrophoresis·Ken-ichi OhnoAndreas Manz
Mar 25, 2009·Angewandte Chemie·Detlev Belder
Feb 25, 2010·Chemical Society Reviews·Daniel MarkRoland Zengerle
Mar 12, 2010·Journal of the American Society for Mass Spectrometry·Jian PeiRobert T Kennedy
Apr 27, 2010·Angewandte Chemie·Hans H Gorris, David R Walt
Jun 10, 2010·Natural Product Reports·Henning Jacob Jessen, Karl Gademann
Jun 24, 2010·Angewandte Chemie·Ashleigh B ThebergeWilhelm T S Huck
Jan 20, 2011·Analytical Chemistry·Ansgar M HuebnerFlorian Hollfelder
Dec 1, 1998·Analytical Chemistry·D C DuffyG M Whitesides
Dec 20, 2011·Journal of the American Chemical Society·Wei LiEugenia Kumacheva
Feb 22, 2012·Lab on a Chip·Ashleigh B ThebergeAndrew D Griffiths
May 24, 2012·Analytical Chemistry·Peng SongRobert T Kennedy
Jul 14, 2012·Analytical Chemistry·Dmitry MomotenkoHubert H Girault
Oct 24, 2012·Nature Chemistry·Inge L C Buurmans, Bert M Weckhuysen
Nov 28, 2012·Angewandte Chemie·Haakan N Joensson, Helene Andersson Svahn
Apr 6, 2013·Nature Protocols·Linas MazutisJohn A Heyman
Jul 3, 2013·Accounts of Chemical Research·Xiao-Feng YangTao Zhang
Jul 9, 2013·Analytica Chimica Acta·Ying Zhu, Qun Fang
Jul 28, 2013·Journal of the American Chemical Society·Eva M Hensle, Suzanne A Blum
Oct 23, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Ju Hyeon KimSeung-Man Yang
Oct 25, 2013·Nature Chemistry·Katherine S ElviraAndrew J deMello
Nov 10, 2013·Accounts of Chemical Research·Wei WangLiang-Yin Chu

❮ Previous
Next ❯

Citations

Dec 16, 2017·Nature Reviews. Drug Discovery·Gisbert Schneider
Jun 5, 2020·Lab on a Chip·Alexander MendlStefan Löbbecke
Oct 27, 2020·Lab on a Chip·Andrea J PeretzkiDetlev Belder
Dec 5, 2020·Nature Communications·Alexandra C SunCorey R J Stephenson
Apr 30, 2019·Analytical Chemistry·Daniel J Steyer, Robert T Kennedy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.