A Droplet Microfluidic System to Fabricate Hybrid Capsules Enabling Stem Cell Organoid Engineering

Advanced Science
Haitao LiuJianhua Qin

Abstract

Organoids derived from self-organizing stem cells represent a major technological breakthrough with the potential to revolutionize biomedical research. However, building high-fidelity organoids in a reproducible and high-throughput manner remains challenging. Here, a droplet microfluidic system is developed for controllable fabrication of hybrid hydrogel capsules, which allows for massive 3D culture and formation of functional and uniform islet organoids derived from human-induced pluripotent stem cells (hiPSCs). In this all-in-water microfluidic system, an array of droplets is utilized as templates for one-step fabrication of binary capsules relying on interfacial complexation of oppositely charged Na-alginate (NaA) and chitosan (CS). The produced hybrid capsules exhibit high uniformity, and are biocompatible, stable, and permeable. The established system enables capsule production, 3D culture, and self-organizing formation of human islet organoids in a continuous process by encapsulating pancreatic endocrine cells from hiPSCs. The generated islet organoids contain islet-specific α- and β-like cells with high expression of pancreatic hormone specific genes and proteins. Moreover, they exhibit sensitive glucose-stimulated insul...Continue Reading

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Citations

Jan 31, 2021·Lab on a Chip·Zheng AoFeng Guo
Nov 14, 2020·Advanced Healthcare Materials·Sung-Min KangJoong Yull Park
Feb 25, 2021·Nature Reviews. Materials·Moritz Hofer, Matthias P Lutolf
Mar 26, 2021·ACS Applied Materials & Interfaces·Mathias SteinacherEsther Amstad
Jan 27, 2021·Stem Cells·Phillip L Lewis, James M Wells
Jun 23, 2021·Acta Biomaterialia·Seokyoung BangHong Nam Kim
Jul 17, 2021·Advanced Materials·Pengchao ZhangLidong Qin
Jul 6, 2021·Macromolecular Bioscience·Pingli WuGuoming Sun
Oct 13, 2021·Biofabrication·Guocheng FangDayong Jin
Feb 3, 2022·Advanced Science·Yaqing WangJianhua Qin

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Methods Mentioned

BETA
chip
Fluorescence
PCR

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