PMID: 6413075Sep 1, 1983Paper

A Drosophila metabolic gene transcript is alternatively processed

Cell
S HenikoffJ D Kelly

Abstract

We have determined the organization and transcription of a Drosophila DNA segment coding for the purine pathway enzyme, GAR transformylase. Because the transcript encoding this enzyme is very rare, we used a novel method for exon mapping without isolating a cDNA. Our results suggest a long polypeptide having multiple domains, with GAR transformylase at the COOH terminus preceded by an extensive repeat. Part of the same DNA segment specifies a shorter transcript, which consists of the same 5' exons but lacks the last three 3' exons. A polyadenylation signal within an intron allows this single gene to encode two polypeptides. We hypothesize that alternative processing of a transcript is a basis for channeling metabolic intermediates into two different pathways.

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Citations

Dec 19, 1998·Microscopy Research and Technique·E A Cox, A Huttenlocher
Jul 1, 1992·Journal of Molecular Evolution·D V Clark, S Henikoff
Oct 1, 1985·Molecular and Cellular Endocrinology·S G Amara
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S HenikoffD Patterson
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S HenikoffD Patterson
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R P HarveyJ P Lecocq
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D SchildP J Barr
Jul 11, 1985·Nucleic Acids Research·E B Keller, W A Noon
Mar 15, 2014·Current Opinion in Hematology·Edward F PlowTatiana V Byzova
Jan 15, 1985·Virology·C Cladaras, W S Wold
Mar 1, 1984·Cell·C L Moore, P A Sharp
Jul 1, 1984·Current Eye Research·M B Gorin, J Horwitz
Jan 1, 1987·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S Henikoff
May 1, 1984·Molecular and Cellular Biology·S W Cartinhour, G A Herrick
Aug 1, 1984·Molecular and Cellular Biology·S Henikoff, E H Cohen
May 1, 1984·Molecular and Cellular Biology·C MontellA J Berk
Sep 21, 1984·Science·M G RosenfeldR M Evans

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