A Drosophila Smyd4 homologue is a muscle-specific transcriptional modulator involved in development.

PloS One
Elizabeth C Thompson, Andrew A Travers

Abstract

SET and MYND domain (Smyd) proteins are involved in the transcriptional regulation of cellular proliferation and development in vertebrates. However, the in vivo functions and mechanisms by which these proteins act are poorly understood. We have used biochemical and genetic approaches to study the role of a Smyd protein in Drosophila. We identified eleven Drosophila genes that encode Smyd proteins. CG14122 encodes a Smyd4 homologue that we have named dSmyd4. dSmyd4 repressed transcription and recruited class I histone deacetylases (HDACs). A region of dSmyd4 including the MYND domain interacted directly with approximately 150 amino acids at the N-termini of dHDAC1 and dHDAC3. dSmyd4 interacts selectively with Ebi, a component of the dHDAC3/SMRTER co-repressor complex. During embryogenesis dSmyd4 was expressed throughout the mesoderm, with highest levels in the somatic musculature. Muscle-specific RNAi against dSmyd4 resulted in depletion of the protein and lead to severe lethality. Eclosion is the final moulting stage of Drosophila development when adult flies escape from the pupal case. 80% of dSmyd4 knockdown flies were not able to eclose, resulting in late pupal lethality. However, many aspects of eclosion were still able to...Continue Reading

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Citations

Oct 27, 2011·Journal of Molecular Cell Biology·Mohamed Abu-FarhaJean-François Couture
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Datasets Mentioned

BETA
RE25548
AT24727
HL04910
LD29892
GM14158
LD23745

Methods Mentioned

BETA
pulldown
transfections
immunoprecipitation
PCR
transfection
confocal microscopy

Software Mentioned

BLAST

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