A dual-channel homogeneous aptasensor combining colorimetric with electrochemical strategy for thrombin

Biosensors & Bioelectronics
Yaxing ZhangQingyun Liu

Abstract

In this protocol, a dual-channel homogeneous aptasenor was proposed for protein molecule determination, employing thrombin as target analyte. The colorimetric and electrochemical transducers were combined in a single analytical system for signal readout. In this dual-channel sensing strategy, the G-quadruplex sequence was released and incorporated with hemin to form DNAzyme for naked-eye colorimetric detection. Meanwhile, the hydroxyapatite nanoparticle as signal probe was combined with magnetic nanoparticles to construct sandwich-type structure for generating the electrochemical current when thrombin was present in solution. By introducing two kinds of reporter probes and transducers, this dual-channel sensor produced two different kinds of signal to improve the analytical accuracy and diversity. The results revealed that the dual-channel sensor achieved the quantatitive determination of thrombin with low limit of detection (0.40 fM) and wide range (0.1 fM to 1 nM), which offer a promise for rapid and accurate detection of biomolecule.

Citations

Jun 14, 2021·Biosensors & Bioelectronics·Yaofeng ZhouYonghua Xiong
Nov 14, 2020·Analytical and Bioanalytical Chemistry·Yitong HuangZhenyu Lin

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