A Dual-Circular RNA Signature as a Non-invasive Diagnostic Biomarker for Gastric Cancer

Frontiers in Oncology
Li HanLei Yin

Abstract

Gastric cancer (GC) is the TOP3 leading cause of human mortality in malignant tumors. Notwithstanding, the association between GC and circRNAs is not clear. The purpose of this research was to determine the association between GC progression and circRNAs. The data of circRNAs was obtained from the Gene Expression Omnibus (GEO) database to identify gene, which differentially expressed circRNAs in GC tissues and paired normal tissues. The expression of circRNAs in cancer tissues and normal tissues were tested, and the target circRNA was verified before and after surgery in the plasma. A circRNA-micro(mi)RNA-mRNA competing endogenous RNAs (ceRNAs) network was established, and GO and KEGG analysis are performed. Five candidate circRNAs were identified through bioinformatics analysis. Hsa_circ_0021087 and hsa_circ_0005051 were both downregulated in GC tissues, cells and plasma by RTq-PCR. Additionally, there was a significant difference in the expression of plasma hsa_circ_0021087 in patients with GC at the preoperative and postoperative stages (P < 0.001). Hsa_circ_0021087 also promoted the proliferation of GC cells in vitro. Next, the circRNA-miRNA-mRNA network of hsa_circ_0021087 was predicted, which may be associated with the de...Continue Reading

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Citations

Oct 17, 2020·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Jason R Brown, Arul M Chinnaiyan
Jun 25, 2021·Journal of Clinical Laboratory Analysis·Lipeng Yao, Yaoyao Xie

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Datasets Mentioned

BETA
GSE93541

Methods Mentioned

BETA
biopsies
transfection
PCR
surgical resection

Clinical Trials Mentioned

NCT03170830
NCT02297776

Software Mentioned

CircInteractome
R
TargetScan
GDCRNATools
clusterProfiler
ImageJ
Starbase
GraphPad Prism
Limma
circBank

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