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A dual ELP-tagged split intein system for non-chromatographic recombinant protein purification

Applied Microbiology and Biotechnology

Dec 6, 2012

Changhua ShiD W Wood

Abstract

Self-cleaving elastin-like protein (ELP) tags provide a very promising tool for recombinant protein purification. With this method, the target protein is purified by simple ELP-mediated precipitation steps, followed by self-cleavage and removal of the ELP tag. Unfortunately, however, in...read more

Mentioned in this Paper

Pathologic Cytolysis
ELN gene
Alkalescens-Dispar Group
Chimeric Proteins, Recombinant
Cytokinesis of the Fertilized Ovum
Intein-mediated Protein Splicing Activity
Protein Purification
Purification Aspects
Recombinants
Cytokinesis
1
59
Paper Details
References
  • References22
  • Citations13
123
  • References22
  • Citations13
12

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A dual ELP-tagged split intein system for non-chromatographic recombinant protein purification

Applied Microbiology and Biotechnology

Dec 6, 2012

Changhua ShiD W Wood

PMID: 23212673

DOI: 10.1007/s00253-012-4601-3

Abstract

Self-cleaving elastin-like protein (ELP) tags provide a very promising tool for recombinant protein purification. With this method, the target protein is purified by simple ELP-mediated precipitation steps, followed by self-cleavage and removal of the ELP tag. Unfortunately, however, in...read more

Mentioned in this Paper

Pathologic Cytolysis
ELN gene
Alkalescens-Dispar Group
Chimeric Proteins, Recombinant
Cytokinesis of the Fertilized Ovum
Intein-mediated Protein Splicing Activity
Protein Purification
Purification Aspects
Recombinants
Cytokinesis
1
59

Similar Papers Found In These Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Related Papers

Nature Protocols

Recombinant protein purification by self-cleaving aggregation tag

Nature ProtocolsApril 5, 2007
Wan-Yi WuD W Wood
Nature Methods

Simple bioseparations using self-cleaving elastin-like polypeptide tags

Nature MethodsAugust 3, 2005
Mahmoud Reza BankiD W Wood
Paper Details
References
  • References22
  • Citations13
123
  • References22
  • Citations13
12
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