A dual-genome microarray for the pea aphid, Acyrthosiphon pisum, and its obligate bacterial symbiont, Buchnera aphidicola

BMC Genomics
Alex C C WilsonNancy A Moran

Abstract

The best studied insect-symbiont system is that of aphids and their primary bacterial endosymbiont Buchnera aphidicola. Buchnera inhabits specialized host cells called bacteriocytes, provides nutrients to the aphid and has co-speciated with its aphid hosts for the past 150 million years. We have used a single microarray to examine gene expression in the pea aphid, Acyrthosiphon pisum, and its resident Buchnera. Very little is known of gene expression in aphids, few studies have examined gene expression in Buchnera, and no study has examined simultaneously the expression profiles of a host and its symbiont. Expression profiling of aphids, in studies such as this, will be critical for assigning newly discovered A. pisum genes to functional roles. In particular, because aphids possess many genes that are absent from Drosophila and other holometabolous insect taxa, aphid genome annotation efforts cannot rely entirely on homology to the best-studied insect systems. Development of this dual-genome array represents a first attempt to characterize gene expression in this emerging model system. We chose to examine heat shock response because it has been well characterized both in Buchnera and in other insect species. Our results from th...Continue Reading

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Citations

Aug 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Carlene K ChunMargaret J McFall-Ngai
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Datasets Mentioned

BETA
GSE3742

Methods Mentioned

BETA
PCR

Software Mentioned

SAS
softWoRx Tracker

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