A dual-layer silk fibroin scaffold for reconstructing the human corneal limbus

Biomaterials
Laura J BrayDamien G Harkin

Abstract

Membranes prepared from Bombyx mori silk fibroin have shown potential as a substrate for human limbal epithelial (L-EC) and stromal cell cultivation. Here we present fibroin as a dual-layer construct containing both an epithelium and underlying stroma for corneolimbal reconstruction. We have compared the growth and phenotype of L-EC on non-porous versus porous fibroin membranes. Furthermore, we have compared the growth of limbal mesenchymal stromal cells (L-MSC) in either serum-supplemented medium or the MesenCult-XF(®) culture system within fibroin fibrous mats. The co-culture of L-EC and L-MSC in fibroin dual-layer constructs was also examined. L-EC on porous membranes displayed a squamous monolayer; in contrast, L-EC on non-porous fibroin appeared cuboidal and stratified. Both constructs maintained evidence of corneal phenotype (cytokeratin 3/12) and distribution of ΔNp63(+) progenitor cells. L-MSC cultivated within fibroin fibrous mats in serum-supplemented medium contained less than 64% of cells expressing the characteristic MSC phenotype of CD73(+)CD90(+)CD105(+) after two weeks, compared with over 81% in MesenCult-XF(®) medium. Dual-layer fibroin scaffolds consisting of L-EC and L-MSC maintained a similar phenotype as on...Continue Reading

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Citations

May 3, 2013·Journal of Molecular Histology·Linan GuanPing Liu
Nov 30, 2012·Future Medicinal Chemistry·Damien G Harkin, Traian V Chirila
Sep 21, 2013·Tissue Engineering. Part C, Methods·Hua MeiSophie X Deng
Mar 26, 2014·Current Opinion in Ophthalmology·May Griffith, Damien G Harkin
Nov 30, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Che Man RohainaRuszymah B H Idrus
Feb 20, 2013·Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie·Johannes Menzel-SeveringUrsula Schlötzer-Schrehardt
Dec 15, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Gaoqiang YangJiannan Wang
Jan 21, 2016·Stem Cells International·Michel HaagdorensNadia Zakaria
Sep 11, 2012·Clinical & Experimental Ophthalmology·Damien G HarkinCharles N J McGhee
Jun 24, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Dan SunJiannan Wang
Oct 15, 2013·Cell Proliferation·S GottipamulaR N Seetharam
Oct 10, 2015·Journal of Tissue Engineering and Regenerative Medicine·Audra M A ShadforthDamien G Harkin
Sep 10, 2014·Biotechnology and Applied Biochemistry·Mazaher GholipourmalekabadiHatef Ghasemi Hamidabadi
Jun 4, 2015·Journal of Functional Biomaterials·Shuko SuzukiDamien G Harkin
Dec 2, 2014·Tissue Engineering. Part B, Reviews·Chiara E GhezziDavid L Kaplan
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Jan 18, 2018·Cell Transplantation·Damien G HarkinNadia Zakaria
Jun 11, 2017·Journal of Tissue Engineering and Regenerative Medicine·Emily A GosselinDavid L Kaplan
Jun 18, 2020·Advanced Healthcare Materials·Guoliang WangCheng Li
Jul 27, 2017·Scientific Reports·Julia Fernández-PérezLaura J Bray
Jul 6, 2019·Current Ophthalmology Reports·Ghasem YazdanpanahAli R Djalilian
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Apr 20, 2018·Physical Chemistry Chemical Physics : PCCP·Yanchen HanXin Chen
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Apr 4, 2021·Pharmaceutics·Mahdi YonesiDaniel González-Nieto
Nov 10, 2020·Experimental Eye Research·Jorge L Alió Del BarrioJorge L Alió
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Jan 19, 2021·ACS Biomaterials Science & Engineering·Linli HuXin Chen
Aug 13, 2018·ACS Biomaterials Science & Engineering·Zhongchun ChenXia Zhao

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