A Dual-Response Fluorescent Probe Reveals the H2 O2 -Induced H2 S Biogenesis through a Cystathionine β-Synthase Pathway

Chemistry : a European Journal
Long YiZhen Xi

Abstract

The two signaling molecules H2 S and H2 O2 play key roles in maintaining intracellular redox homeostasis. The biological relationship between H2 O2 and H2 S remains largely unknown in redox biology. In this study, we rationally designed and synthesized single- and dual-response fluorescent probes for detecting both H2 O2 and H2 S in living cells. The dual-response probe was shown to be capable of mono- and dual-detection of H2 O2 and H2 S selectively and sensitively. Detailed bioimaging studies based on the probes revealed that both exogenous and endogenous H2 O2 could induce H2 S biogenesis in living cells. By using gene-knockdown techniques with bioimaging, the H2 S biogenesis was found to be majorly cystathionine β-synthase (CBS)-dependent. Our finding shows the first direct evidence on the biological communication between H2 O2 (ROS) and H2 S (RSS) in vivo.

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Citations

Mar 27, 2018·Chemical Communications : Chem Comm·Changyu ZhangLong Yi
Jun 18, 2019·Chemical Society Reviews·Yongkang YueCaixia Yin
Nov 14, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jiawen ZhuWenying Yu
Jun 12, 2020·Chemical Communications : Chem Comm·Huawei NiuYufen Zhao
Mar 10, 2016·Chembiochem : a European Journal of Chemical Biology·Runyu WangZhen Xi
Aug 2, 2020·Chemistry : a European Journal·Ting ChenBang-Ping Jiang
Oct 15, 2020·Analytical Methods : Advancing Methods and Applications·Songtao CaiXianshun Zeng
May 4, 2018·Journal of the American Chemical Society·Clive Yik-Sham ChungChristopher J Chang

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