A Dual Role for Corneal Dendritic Cells in Herpes Simplex Keratitis: Local Suppression of Corneal Damage and Promotion of Systemic Viral Dissemination

PloS One
Kai HuPedram Hamrah

Abstract

The cornea is the shield to the foreign world and thus, a primary site for peripheral infections. However, transparency and vision are incompatible with inflammation and scarring that may result from infections. Thus, the cornea is required to perform a delicate balance between fighting infections and preserving vision. To date, little is known about the specific role of antigen-presenting cells in viral keratitis. In this study, utilizing an established murine model of primary acute herpes simplex virus (HSV)-1 keratitis, we demonstrate that primary HSV keratitis results in increased conventional dendritic cells (cDCs) and macrophages within 24 hours after infection. Local depletion of cDCs in CD11c-DTR mice by subconjuntival diphtheria toxin injections, led to increased viral proliferation, and influx of inflammatory cells, resulting in increased scarring and clinical keratitis. In addition, while HSV infection resulted in significant corneal nerve destruction, local depletion of cDCs resulted in a much more severe loss of corneal nerves. Further, local cDC depletion resulted in decreased corneal nerve infection, and subsequently decreased and delayed systemic viral transmission in the trigeminal ganglion and draining lymph n...Continue Reading

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Citations

Jan 26, 2016·The Ocular Surface·James L FunderburghYiqin Du
Feb 29, 2020·International Ophthalmology·Dimitrios KalogeropoulosChris Kalogeropoulos
Jul 28, 2020·Progress in Retinal and Eye Research·Arsia JamaliPedram Hamrah
Mar 11, 2021·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Sana ArshadNicole A Carnt
Aug 9, 2016·The American Journal of Pathology·Ana J Chucair-ElliottDaniel J J Carr
Oct 9, 2021·Clinical Science·Laura MarongiuFrancesca Granucci

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Methods Mentioned

BETA
PCR
transgenic

Software Mentioned

Neuron J
SPSS
Imaris
Image J

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