PMID: 8970954Dec 1, 1996Paper

A dual role of the putative RNA dimerization initiation site of human immunodeficiency virus type 1 in genomic RNA packaging and proviral DNA synthesis

Journal of Virology
J C PaillartC Ehresmann

Abstract

In retroviruses, the genomic RNA is in the form of a 60S-70S complex composed of two identical genome-length RNA molecules tightly associated through numerous interactions. A major interaction, called the dimer linkage structure, has been found near the RNA 5' end and is probably involved in the control of translation, packaging, and recombination during proviral DNA synthesis. Recently, a small sequence corresponding to a stem-loop structure located in the 5' leader of human immunodeficiency virus type 1 (HIV-1) RNA was found to be required for the initiation of HIV-1 RNA dimerization in vitro and named the dimerization initiation site (E. Skripkin, J.-C. Paillart, R. Marquet, B. Ehresmann, and C. Ehresmann, Proc. Natl. Acad. Sci. USA 91: 4945-4949, 1994). To investigate the possible role of this 5' stem-loop in HIV-1 virion formation and infectivity, four mutant viruses were generated and analyzed in vivo. Results show that deletion of the stem-loop structure reduces infectivity by a factor of 10(3) whereas loop substitutions cause a decrease of 10- to 100-fold. The level of genomic RNA packaging was found to be decreased fivefold in mutants virions containing the stem-loop deletion and only twofold in the loop-substituted vi...Continue Reading

References

Nov 1, 1975·Journal of Virology·C M Stoltzfus, P N Snyder
May 11, 1992·Journal of Virology·G L Buchschacher, A T Panganiban
Jun 1, 1992·Virology·T HayashiH Shibuta
Nov 30, 1990·Science·W S Hu, H M Temin
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·E CanaaniP Duesberg
Jun 14, 1967·Journal of Molecular Biology·B Hirt
Dec 8, 1995·Journal of Molecular Biology·J L DarlixB P Roques
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·H M Temin

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Citations

Jun 17, 1998·Nature Structural Biology·A MujeebT G Parslow
Nov 15, 2016·RNA Biology·Serena BernacchiJean-Christophe Paillart
Dec 24, 2016·Viruses·Sarah C Keane, Michael F Summers
Nov 30, 2002·The Journal of Biological Chemistry·Truus E M Abbink, Ben Berkhout
Jun 15, 2004·The Journal of Biological Chemistry·Valérie GoldschmidtRoland Marquet
Sep 20, 2005·The Journal of Biological Chemistry·Serena BernacchiPhilippe Dumas
Jan 1, 2000·Journal of Biomolecular Structure & Dynamics·T L JamesA Mujeeb
Aug 14, 2012·Journal of Biomolecular Structure & Dynamics·Taejin Kim, Bruce A Shapiro
May 13, 2014·Nucleic Acids Research·Hansini MundigalaDavid Rueda
Jul 6, 2014·Nature Communications·Ekram W Abd El-WahabRoland Marquet
Mar 27, 2001·Journal of Virology·D CainM O McClure
Dec 18, 2001·The Journal of Biological Chemistry·Jean-Christophe PaillartRoland Marquet
Mar 9, 2002·Journal of Virology·Melinda Butsch, Kathleen Boris-Lawrie
Apr 15, 2003·Antisense & Nucleic Acid Drug Development·Emily L DeerJ Stephen Lodmell
Mar 9, 2002·Journal of Virology·Hinh Ly, Tristram G Parslow
Jan 22, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mihaela-Rita Mihailescu, John P Marino
Sep 29, 2004·Journal of Virology·Catherine S HibbertAlan Rein
Sep 10, 2004·The Journal of Biological Chemistry·Jean-Christophe PaillartRoland Marquet
Jun 19, 2009·Expert Opinion on Biological Therapy·Virendra N PandeyBinay Chaubey
May 21, 2004·Nature Reviews. Microbiology·Jean-Christophe PaillartJohnson Mak
Dec 25, 2008·Nucleic Acids Research·Cameron P KeatingJohnson Mak
Jun 10, 2016·Nucleic Acids Research·Mireia FerrerMarylène Mougel
Feb 14, 2012·Nucleic Acids Research·Jun-ichi SakuragiHironori Sato
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Sarah C KeaneMichael F Summers

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