A duplex real-time reverse transcriptase polymerase chain reaction assay for the detection of St. Louis encephalitis and eastern equine encephalitis viruses.

Diagnostic Microbiology and Infectious Disease
Rene HullNorma P Tavakoli

Abstract

A duplex TaqMan real-time reverse transcriptase polymerase chain reaction (PCR) assay was developed for the detection of St. Louis encephalitis virus (SLEV) and eastern equine encephalitis virus (EEEV), for use in human and vector surveillance. The respective targets selected for the assay were the conserved NS5 and E1 genes of the 2 viruses. Because of the insufficient number of NS5 sequences from SLEV strains in the GenBank database, we determined the sequence of an approximately 1-kb region for each of 25 strains of SLEV to select primers and probes in a conserved region. Our assay has a sensitivity of 5 gene copies (gc)/reaction for EEEV and 10 gc/reaction for SLEV, and its performance is linear for at least 6 log(10) gc. The assay is specific and detected all strains of SLEV (69) and EEEV (12) that were tested. An internal control ensures detection of efficient nucleic acid extraction and possible PCR inhibition.

References

Jun 26, 1997·The New England Journal of Medicine·R L DeresiewiczA A Zamani
Oct 3, 2001·Journal of Virology·A M PowersS C Weaver
Jan 9, 2003·Journal of Clinical Microbiology·Amy J LambertRobert S Lanciotti
Jun 10, 2004·Transactions of the Royal Society of Tropical Medicine and Hygiene·Roberta V M BronzoniLuiz Tadeu M Figueiredo
Feb 8, 2005·Journal of Clinical Microbiology·Roberta Vieira de Morais BronzoniLuiz Tadeu Moraes Figueiredo
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar

❮ Previous
Next ❯

Citations

Mar 23, 2011·Journal of Virological Methods·Marcio R T NunesPedro F C Vasconcelos
Oct 5, 2011·Parasites & Vectors·Marieta BraksHein Sprong
Jun 29, 2016·SpringerPlus·Daniel García-RuízDarwin Elizondo-Quiroga
Jul 28, 2007·Virology·Mark W EshooDavid J Ecker
Aug 26, 2016·Archives of Virology·Marilia Farignoli RomeiroLuiz Tadeu Moraes Figueiredo
Oct 21, 2011·Journal of Medical Virology·Michelle DupuisNorma P Tavakoli
Sep 28, 2017·PLoS Neglected Tropical Diseases·Camila LorenzLincoln Suesdek
Mar 24, 2021·Emerging Infectious Diseases·Kimberly A ThompsonMatti Kiupel

❮ Previous
Next ❯

Datasets Mentioned

BETA
EF568607
EF158050
EU341596
EEE71-20686
GML
903369

Methods Mentioned

BETA
PCR
nucleic
electrophoresis
nucleic acid extraction

Software Mentioned

MEGA
Primer Express

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.