A dynamical model of the spindle position checkpoint.

Molecular Systems Biology
Ayse Koca CaydasiBashar Ibrahim

Abstract

The orientation of the mitotic spindle with respect to the polarity axis is crucial for the accuracy of asymmetric cell division. In budding yeast, a surveillance mechanism called the spindle position checkpoint (SPOC) prevents exit from mitosis when the mitotic spindle fails to align along the mother-to-daughter polarity axis. SPOC arrest relies upon inhibition of the GTPase Tem1 by the GTPase-activating protein (GAP) complex Bfa1-Bub2. Importantly, reactions signaling mitotic exit take place at yeast centrosomes (named spindle pole bodies, SPBs) and the GAP complex also promotes SPB localization of Tem1. Yet, whether the regulation of Tem1 by Bfa1-Bub2 takes place only at the SPBs remains elusive. Here, we present a quantitative analysis of Bfa1-Bub2 and Tem1 localization at the SPBs. Based on the measured SPB-bound protein levels, we introduce a dynamical model of the SPOC that describes the regulation of Bfa1 and Tem1. Our model suggests that Bfa1 interacts with Tem1 in the cytoplasm as well as at the SPBs to provide efficient Tem1 inhibition.

References

Jan 1, 1991·Methods in Enzymology·F Sherman
Oct 8, 1998·Molecular Biology of the Cell·S L JaspersenD O Morgan
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·R Li
Feb 9, 2000·Molecular Cell Biology Research Communications : MCBRC·R CenamorM Sánchez
Jun 6, 2000·Current Biology : CB·S L Jaspersen, D O Morgan
Aug 19, 2000·Molecular Cell·G PereiraE Schiebel
Nov 9, 2000·Current Biology : CB·A F Straight, C M Field
Sep 21, 2001·Molecular and Cellular Biology·F C LucaM Winey
Oct 13, 2001·Molecular Biology of the Cell·R Visintin, A Amon
Apr 6, 2002·Current Opinion in Microbiology·Antonio Casamayor, Michael Snyder
Apr 24, 2002·The Journal of Cell Biology·Gislene PereiraElmar Schiebel
Jun 6, 2002·The Journal of Biological Chemistry·Marco GeymonatSteven G Sedgwick
Aug 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jörg WiedenmannG Ulrich Nienhaus
Dec 4, 2002·Cell Cycle·Fenghua Hu, Stephen J Elledge
Mar 15, 2003·The Journal of Biological Chemistry·Marco GeymonatSteven G Sedgwick
Jun 5, 2003·The Journal of Cell Biology·Satoshi YoshidaAkio Toh-e
Oct 17, 2003·Nature·Sina GhaemmaghamiJonathan S Weissman
Jan 14, 2004·Molecular Biology of the Cell·Jeffrey N MolkKerry Bloom
Jan 22, 2004·The Journal of Cell Biology·Thomas Höfken, Elmar Schiebel
Jun 1, 2004·Molecular Biology of the Cell·Katherine C ChenJohn J Tyson
Oct 29, 2004·Molecular and Cellular Biology·Jung-Eun ParkKyung S Lee
Dec 1, 2004·Annual Review of Genetics·Frank Stegmeier, Angelika Amon
Apr 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Andreas DoncicNaama Barkai
Jul 26, 2005·Molecular Cell·Gislene Pereira, Elmar Schiebel
Jul 26, 2005·Molecular Cell·Katharine E D'AquinoAngelika Amon
May 23, 2006·Nature Cell Biology·Ajit P JoglekarEdward D Salmon
Oct 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Richard P Sear, Martin Howard
Apr 12, 2007·Nature Reviews. Molecular Cell Biology·Andrea Musacchio, Edward D Salmon
Jul 31, 2007·Journal of Theoretical Biology·Attila TóthBéla Novák
Dec 25, 2007·Methods in Cell Biology·Ajit P JoglekarKerry S Bloom

❮ Previous
Next ❯

Citations

Jul 23, 2013·PLoS Computational Biology·Archana BajpaiAttila Csikász-Nagy
Oct 17, 2012·PloS One·Peter KreyssigPeter Dittrich
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Hyeon-Su RoKyung S Lee
Oct 23, 2014·International Journal of Molecular Sciences·Bashar Ibrahim, Richard Henze
Feb 28, 2015·Progress in Biophysics and Molecular Biology·Bashar Ibrahim
Apr 9, 2013·Progress in Biophysics and Molecular Biology·Sergej TschernyschkowBashar Ibrahim
Sep 1, 2018·G3 : Genes - Genomes - Genetics·Jenna WhalenAnupama Seshan
Feb 20, 2020·Molecular Biology of the Cell·Ian W CampbellAngelika Amon
Nov 23, 2017·Mathematical Biosciences and Engineering : MBE·Bashar Ibrahim
Nov 30, 2018·Physical Biology·M AkiyamaR Kobayashi
Nov 13, 2020·PLoS Biology·Rowan S M HowellAttila Csikász-Nagy
Feb 4, 2021·Bioinformatics·Luca CardelliTabea Waizmann

❮ Previous
Next ❯

Datasets Mentioned

BETA
MODEL1202090001
MODEL1202090002
MODEL1202090003

Methods Mentioned

BETA
GTPase
nucleotide exchange
fluorescence microscopy
and

Software Mentioned

softWoRx
SPOC
image J
matlab
Metamorph
Image J ( NIH )
Excel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.