A dynamin-actin interaction is required for vesicle scission during endocytosis in yeast

Current Biology : CB
Sarah E PalmerKathryn R Ayscough

Abstract

Actin is critical for endocytosis in yeast cells, and also in mammalian cells under tension. However, questions remain as to how force generated through actin polymerization is transmitted to the plasma membrane to drive invagination and scission. Here, we reveal that the yeast dynamin Vps1 binds and bundles filamentous actin. Mutational analysis of Vps1 in a helix of the stalk domain identifies a mutant RR457-458EE that binds actin more weakly. In vivo analysis of Vps1 function demonstrates that the mutation disrupts endocytosis but not other functions of Vps1 such as vacuolar trafficking or peroxisome fission. The mutant Vps1 is stably expressed in cells and co-localizes with the endocytic reporters Abp1 and the amphiphysin Rvs167. Detailed analysis of individual endocytic patch behavior indicates that the mutation causes aberrant movements in later stages of endocytosis, consistent with a scission defect. Ultrastructural analysis of yeast cells using electron microscopy reveals a significant increase in invagination depth, further supporting a role for the Vps1-actin interaction during scission. In vitro analysis of the mutant protein demonstrates that--like wild-type Vps1--it is able to form oligomeric rings, but, criticall...Continue Reading

References

Aug 29, 2002·Nature Cell Biology·Christien J MerrifieldWolfhard Almers
Jul 19, 2003·The Biochemical Journal·Steven J WinderKathryn R Ayscough
May 26, 2005·Biochimica Et Biophysica Acta·Katherine Bowers, Tom H Stevens
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jian LiuGeorge Oster
Mar 18, 2009·Cellular and Molecular Life Sciences : CMLS·Alastair S RobertsonKathryn R Ayscough
Jun 10, 2009·The Journal of Cell Biology·Helen E BurstonElizabeth Conibear
Jul 15, 2009·Nature Cell Biology·Soheil Aghamohammadzadeh, Kathryn R Ayscough
Sep 16, 2010·Journal of Cell Science·Iwona I Smaczynska-de RooijKathryn R Ayscough
Oct 12, 2010·The EMBO Journal·Changkyu GuSanja Sever
May 24, 2011·Annual Review of Cell and Developmental Biology·Sandra L Schmid, Vadim A Frolov
Aug 16, 2011·Nature Cell Biology·Steeve BoulantTomas Kirchhausen
Oct 25, 2011·Trends in Cell Biology·Jasper Weinberg, David G Drubin
Nov 9, 2011·The Journal of Cell Biology·Derek C ProsserBeverly Wendland
Nov 16, 2011·Traffic·Iwona I Smaczynska-de RooijKathryn R Ayscough
Dec 23, 2011·Molecular Biology of the Cell·Susheela Y CarrollDavid G Drubin
Mar 1, 2012·Traffic·Anne-Cécile DurieuxMarc Bitoun
Aug 8, 2012·The Journal of Cell Biology·Karen E MoreiraPeter Walter
Aug 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Michal SkruznyMarko Kaksonen
Jan 8, 2013·Current Biology : CB·Agnieszka N UrbanekKathryn R Ayscough
Apr 24, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Laura BriñasMarc Bitoun
Sep 26, 2013·Frontiers in Endocrinology·Arlek M González-JamettAna María Cárdenas
Jun 4, 2014·The Journal of Cell Biology·Alexandre GrassartDavid G Drubin

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Citations

Oct 20, 2015·Communicative & Integrative Biology·Sarah E PalmerKathryn R Ayscough
Feb 6, 2016·Traffic·Florence Marie-AnaïsFlorence Niedergang
Apr 12, 2016·Biology of the Cell·Kyoungtae Kim, Shiva Kumar Goud Gadila
Apr 17, 2016·Journal of Cell Science·Rebecca LuYidi Sun
Feb 8, 2018·Nature Reviews. Molecular Cell Biology·Marko Kaksonen, Aurélien Roux
Aug 26, 2016·Molecular Biology of the Cell·Anna L HatchHenry N Higgs
Nov 5, 2017·The Journal of Biological Chemistry·Daniel J SchneiderMarc Peters-Golden
Dec 30, 2015·Molecular and Cellular Biology·Iwona I Smaczynska-de RooijKathryn R Ayscough
Jul 20, 2016·Journal of the American Society of Nephrology : JASN·Changkyu GuSanja Sever
Jun 9, 2020·Proteins·Pragyan Parimita Rath, Samudrala Gourinath
Jul 1, 2020·Frontiers in Immunology·Valentin Jaumouillé, Clare M Waterman
Apr 22, 2018·Biochemical Society Transactions·Claudia Hinze, Emmanuel Boucrot
Jan 29, 2021·Life·Olivia L McGovernVern B Carruthers

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Methods Mentioned

BETA
fluorescence microscopy
GTPase
electron microscopy
circular dichroism

Software Mentioned

NIS Elements
GraphPad Prism

Related Concepts

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