Aβ Extraction from Murine Brain Homogenates

Bio-protocol
Brad T Casali, Gary E Landreth

Abstract

This protocol details beta-amyloid (Aβ) extraction from transgenic murine brain homogenates. Specifically, mechanical homogenization of brain tissue and sequential extraction of both soluble and insoluble proteins are detailed. DEA extracts soluble proteins, such as Aβ isoforms and APP. Formic acid enables extraction of insoluble protein aggregates, such as Aβ isoforms associated with plaques. This procedure produces soluble and insoluble extracts that are amenable to analysis of Aβ species via western blotting and/or enzyme-linked immunosorbent assays (ELISAs), and these results help assess amyloidogenic burden in animals.

References

Dec 31, 2011·Neurobiology of Aging·Jose Morales-CorralizaPaul M Mathews
Jun 19, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Brad T CasaliGary E Landreth

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Citations

Jun 3, 2018·Molecular Neurodegeneration·Paul J Cheng-HathawayGary E Landreth
Feb 20, 2021·Molecular Neurobiology·Ana Catarina Rodrigues-NevesAntónio Francisco Ambrósio
Jun 23, 2021·The Journal of Experimental Medicine·Peng XuPaul Greengard
Aug 10, 2021·Frontiers in Aging Neuroscience·Adrian L OblakBruce T Lamb
Nov 13, 2021·Journal of Neurochemistry·Martyna M MatuszykStephen B Wharton

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