A facile method for high-throughput co-expression of protein pairs.

Molecular & Cellular Proteomics : MCP
Andrei AlexandrovElizabeth J Grayhack

Abstract

We developed a method to co-express protein pairs from collections of otherwise identical Escherichia coli plasmids expressing different ORFs by incorporating a 61-nucleotide sequence (LINK) into the plasmid to allow generation of tandem plasmids. Tandem plasmids are formed in a ligation-independent manner, propagate efficiently, and produce protein pairs in high quantities. This greatly facilitates co-expression for structural genomics projects that produce thousands of clones bearing identical origins and antibiotic markers.

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Citations

Oct 31, 2013·Biotechnology Letters·Alejandro Hochkoeppler
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