A facile quantitative assay for viral particle genesis reveals cooperativity in virion assembly and saturation of an antiviral protein.

Virology
Shalini S YadavPaul D Bieniasz

Abstract

Conventional assays of viral particle assembly and release are time consuming and laborious. We have developed an enzymatic virus-like particle (VLP) genesis assay that rapid and quantitative and is also versatile and applicable to diverse viruses including HIV-1 and Ebola virus. Using this assay, which has a dynamic range of several orders of magnitude, we show that the efficiency of VLP assembly and release, i.e., the fraction of the expressed protein that is assembled into extracellular particles, is dependent on the absolute level of expression of either HIV-1 Gag or Ebola virus VP40. We also demonstrate that the activity of the antiviral factor tetherin is dependent on the level of HIV-1 Gag expression and the numbers of VLPs generated, and appears to become saturated as these parameters are increased.

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Citations

Nov 29, 2013·Expert Opinion on Therapeutic Targets·Robert V Stahelin
Oct 27, 2012·Virus Research·Ina P O'CarrollAlan Rein
May 2, 2014·Frontiers in Microbiology·Daniel Sauter
Sep 21, 2012·Journal of Virology·Ina P O'CarrollAlan Rein
Jan 6, 2017·Journal of Virology·Jordan T Becker, Nathan M Sherer
May 26, 2017·Journal of Virology·Kari A DilleyWei-Shau Hu

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