A facile way to tune mechanical properties of artificial elastomeric proteins-based hydrogels

Langmuir : the ACS Journal of Surfaces and Colloids
Jie Fang, Hongbin Li

Abstract

Protein-based hydrogels have attracted considerable interests due to their potential applications in biomedical engineering and material sciences. Using a tandem modular protein (GB1)(8) as building blocks, we have engineered chemically cross-linked hydrogels via a photochemical cross-linking strategy, which is based on the cross-linking of two adjacent tyrosine residues into dityrosine adducts. However, because of the relatively low reactivity of tyrosine residues in GB1, (GB1)(8)-based hydrogels exhibit poor mechanical properties. Here, we report a Bolton-Hunter reagent-based, facile method to improve and tune the mechanical properties of such protein-based hydrogels. Using Bolton-Hunter reagent, we can derivatize lysine residues with phenolic functional groups to modulate the phenolic (tyrosine-like) content of (GB1)(8). We show that hydrogels made from derivatized (GB1)(8) with increased phenolic content show significantly improved mechanical properties, including improved Young's modulus, breaking modulus as well as reduced swelling. These results demonstrate the great potential of this derivatization method in constructing protein-based biomaterials with desired macroscopic mechanical properties.

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Citations

Oct 24, 2013·Biomacromolecules·Renay S-C SuJulie C Liu
Aug 31, 2013·Physical Chemistry Chemical Physics : PCCP·Toni HoffmannLorna Dougan
Dec 20, 2013·Nature Communications·Jie FangHongbin Li
Dec 29, 2015·Small·Hongbin LiJunqiu Liu
May 12, 2015·Macromolecular Materials and Engineering·Farees SaqlainJorge Alegre-Cebollada
Aug 2, 2016·Biomacromolecules·Xiaoye GaoHongbin Li
Apr 2, 2020·Frontiers in Chemistry·Christopher P CampMinkyu Kim
Dec 1, 2019·Nature Communications·Luai R Khoury, Ionel Popa
Sep 15, 2020·Biomedical Materials·Danielle L HeichelKelly A Burke
Oct 7, 2015·Advanced Functional Materials·David W HowellSarah E Bondos
Jun 5, 2020·Science Advances·Luai R KhouryIonel Popa
Jul 3, 2021·Nanomaterials·Carla Huerta-López, Jorge Alegre-Cebollada
Dec 12, 2016·ACS Biomaterials Science & Engineering·Benjamin P PartlowDavid L Kaplan

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