A factor from Trypanosoma cruzi induces repetitive cytosolic free Ca2+ transients in isolated primary canine cardiac myocytes.

Infection and Immunity
S C BarrR F Gilmour

Abstract

An unusual 120-kDa alkaline peptidase contained in a trypomastigote soluble fraction (TSF) of Trypanosoma cruzi is associated with the induction of repetitive Ca2+ transients and subsequent invasion by the parasite of a number of mammalian cell lines, including tissue culture L6E2 myoblasts (B. A. Burleigh and N. W. Andrews, J. Biol. Chem. 270:5172-5180, 1995; S. N. J. Moreno, J. Silva, A. E. Vercesi, and R. Docampo, J. Exp. Med. 180:1535-1540, 1994; A. Rodríguez, M. G. Rioult, A. Ora, and N. W. Andrews, J. Cell Biol. 129:1263-1273, 1995; I. Tardieux, M. H. Nathanson, and N. W. Andrews, J. Exp. Med. 179:1017-1022, 1994). Using single cell spectrofluorometry and whole-cell patch clamping, we show that TSF produces rapid repetitive cytosolic Ca2+ transients (each associated with cell contraction) in primary cardiac myocytes isolated from dogs. The response of myocytes to TSF was dose dependent in that increasing numbers of cells responded to increasing concentrations of TSF. The TSF-induced Ca2+ transients could be obliterated when TSF was heated or treated with trypsin or the protease inhibitor leupeptin. Aprotinin, pepstatin A, and E-64 did not affect TSF activity. The TSF-induced Ca2+ transients and trypomastigote cell invasio...Continue Reading

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Citations

Aug 26, 2003·Memórias do Instituto Oswaldo Cruz·Luciana R GarzoniMaria de Nazareth S Leal de Meirelles
Apr 23, 2013·Traffic·Claire E ButlerKevin M Tyler
Apr 11, 2020·PLoS Neglected Tropical Diseases·Alfredo MijaresJosé R Lopez
Mar 15, 2006·Anais Da Academia Brasileira De Ciências·Nobuko Yoshida
May 23, 2003·The Journal of Parasitology·S C BarrJ S Chandler
Sep 7, 2020·Cell Biochemistry and Function·Roberta LattanziRossella Miele
Dec 7, 2013·PloS One·Bria M CoatesConrad L Epting
Nov 17, 2017·The American Journal of Tropical Medicine and Hygiene·Iván BarríaJosé L Vega
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Jan 30, 1999·International Journal for Parasitology·N Rodriguez-CabezasA Osuna
Jul 3, 2021·Life·Oscar Hernán Rodríguez-BejaranoManuel Alfonso Patarroyo

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