Abstract
An unusual 120-kDa alkaline peptidase contained in a trypomastigote soluble fraction (TSF) of Trypanosoma cruzi is associated with the induction of repetitive Ca2+ transients and subsequent invasion by the parasite of a number of mammalian cell lines, including tissue culture L6E2 myoblasts (B. A. Burleigh and N. W. Andrews, J. Biol. Chem. 270:5172-5180, 1995; S. N. J. Moreno, J. Silva, A. E. Vercesi, and R. Docampo, J. Exp. Med. 180:1535-1540, 1994; A. Rodríguez, M. G. Rioult, A. Ora, and N. W. Andrews, J. Cell Biol. 129:1263-1273, 1995; I. Tardieux, M. H. Nathanson, and N. W. Andrews, J. Exp. Med. 179:1017-1022, 1994). Using single cell spectrofluorometry and whole-cell patch clamping, we show that TSF produces rapid repetitive cytosolic Ca2+ transients (each associated with cell contraction) in primary cardiac myocytes isolated from dogs. The response of myocytes to TSF was dose dependent in that increasing numbers of cells responded to increasing concentrations of TSF. The TSF-induced Ca2+ transients could be obliterated when TSF was heated or treated with trypsin or the protease inhibitor leupeptin. Aprotinin, pepstatin A, and E-64 did not affect TSF activity. The TSF-induced Ca2+ transients and trypomastigote cell invasio...Continue Reading
References
Jun 1, 1976·The Journal of Experimental Medicine·N Nogueira, Z Cohn
Dec 24, 1992·Cell·I TardieuxN W Andrews
Aug 1, 1992·The Journal of Experimental Medicine·B F HallN W Andrews
Jul 1, 1991·Circulation Research·S A MorrisJ P Bilezikian
Nov 1, 1990·Journal of Cellular Physiology·S A MorrisP B Gordon
May 10, 1991·Science·M I SimonN Gautam
Feb 1, 1991·Infection and Immunity·S SchenkmanV Nussenzweig
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
Sep 1, 1990·The Journal of protozoology·L Choromanski, R E Kuhn
Jul 1, 1986·Journal of Molecular and Cellular Cardiology·R W TsienM C Nowycky
Feb 1, 1985·The Journal of General Physiology·A Fabiato
Apr 1, 1988·Circulation Research·S A MorrisM Wittner
Oct 7, 1988·Cell·S SchenkmanE S Robbins
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G CallewaertM Morad
Dec 1, 1987·Experimental Parasitology·N W AndrewsV Nussenzweig
Aug 1, 1982·The Journal of Cell Biology·R Y TsienT J Rink
May 1, 1982·The Journal of protozoology·N W Andrews, W Colli
Jun 1, 1995·The Journal of Cell Biology·A RodríguezN W Andrews
Mar 10, 1995·The Journal of Biological Chemistry·B A Burleigh, N W Andrews
Oct 1, 1994·The Journal of Experimental Medicine·S N MorenoR Docampo
Mar 1, 1994·The Journal of Experimental Medicine·I TardieuxN W Andrews
Nov 1, 1993·Cell Calcium·L F Jaffe
Oct 1, 1993·Cardiovascular Research·D M BersR A Bassani
Aug 26, 1993·Nature·C Randriamampita, R Y Tsien
Mar 1, 1993·The Journal of Cell Biology·A J HuangS C Silverstein
Citations
Aug 26, 2003·Memórias do Instituto Oswaldo Cruz·Luciana R GarzoniMaria de Nazareth S Leal de Meirelles
Nov 17, 2010·Antimicrobial Agents and Chemotherapy·Daniel AdesseLuciana R Garzoni
Apr 23, 2013·Traffic·Claire E ButlerKevin M Tyler
Apr 11, 2020·PLoS Neglected Tropical Diseases·Alfredo MijaresJosé R Lopez
Feb 10, 1997·The Journal of Cell Biology·B A BurleighN W Andrews
Mar 15, 2006·Anais Da Academia Brasileira De Ciências·Nobuko Yoshida
May 23, 2003·The Journal of Parasitology·S C BarrJ S Chandler
Jul 31, 2013·Cardiovascular Research·Elspeth B ElliottChristopher M Loughrey
Sep 7, 2020·Cell Biochemistry and Function·Roberta LattanziRossella Miele
Jan 20, 2000·Infection and Immunity·P M ManqueN Yoshida
Dec 7, 2013·PloS One·Bria M CoatesConrad L Epting
Nov 17, 2017·The American Journal of Tropical Medicine and Hygiene·Iván BarríaJosé L Vega
Jun 16, 2018·Basic Research in Cardiology·Jan BeckendorfJohannes Backs
Jun 27, 1998·Experimental Parasitology·S FavoretoN Yoshida
Jan 30, 1999·International Journal for Parasitology·N Rodriguez-CabezasA Osuna
Jul 3, 2021·Life·Oscar Hernán Rodríguez-BejaranoManuel Alfonso Patarroyo