PMID: 7016920Jun 1, 1981Paper

A factor X-activating cysteine protease from malignant tissue

The Journal of Clinical Investigation
S G Gordon, B A Cross

Abstract

A proteolytic procoagulant has been identified in extracts of human and animal tumors and in cultured malignant cells. It directly activated Factor X but its similarity to other Factor S-activating serine proteases was not clear. This study describes work done to determine whether this enzyme, cancer procoagulant, is a serine or cysteine protease. Purified cancer procoagulant from rabbit V2 carcinoma was bound to a p-chloromercurialbenzoate-agarose affinity column and was eluted with dithiothreitol. The initiation of recalcified, citrated plasma coagulation activity by cancer procoagulant was inhibited by 5 mM diisopropylfluorophosphate, 1 mM phenylmethylsulfonylfluoride, 0.1 mM HgCl2, and 1 mM iodoacetamide. Activity was restored in the diisopropylfluorophosphate-, phenylmethylsulfonylfluoride-, and HgCl2-inhibited samples by 5 mM dithiothreitol; iodoacetamide inhibition was irreversible. Russell's viper venom, a control Factor X-activating serine protease, was not inhibited by either 0.1 mM HgCl2 or 1 mM iodoacetamide. The direct activation of Factor X by cancer procoagulant in a two-stage assay was inhibited by diisopropylfluorophosphate and iodoacetamide. Diisopropylfluorophosphate inhibits serine proteases, and an undefine...Continue Reading

References

Nov 26, 1976·Biochimica Et Biophysica Acta·Y FurukawaK Hayashi
Oct 28, 1976·Biochimica Et Biophysica Acta·J McdonaghR P Mcdonagh
Jan 1, 1976·Analytical Biochemistry·A Bensadoun, D Weinstein
Mar 8, 1966·Biochemical and Biophysical Research Communications·M EbataK T Asunobu
May 1, 1980·Thrombosis Research·M ColucciN Semeraro
Dec 13, 1963·Biochimica Et Biophysica Acta·G M PURCELL, M I BARNHART

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Citations

Sep 1, 1990·American Journal of Hematology·S Nand, H Messmore
Sep 1, 1991·American Journal of Hematology·H A LiebmanJ M Dilorio
Nov 1, 1990·Hematological Oncology·K el-BaruniJ L Francis
Jan 1, 1983·Cancer Metastasis Reviews·H F DvorakA M Dvorak
Jan 1, 1984·Cancer Metastasis Reviews·G J Gasic
Jan 1, 1988·Journal of Cancer Research and Clinical Oncology·M SzczepańskiW Pypno
Nov 1, 1992·Cancer Metastasis Reviews·L V Rao
Nov 1, 1992·Cancer Metastasis Reviews·S G Gordon, M Chelladurai
Mar 1, 1993·World Journal of Surgery·C J Kelly, J M Daly
May 24, 2011·Cancer Chemotherapy and Pharmacology·Søren Astrup Jensen, Jens Benn Sørensen
Aug 1, 1983·Archives of Biochemistry and Biophysics·G D Green, E Shaw
May 1, 1984·Archives of Biochemistry and Biophysics·A K Willingham, J T Matschiner
Oct 1, 1994·Atherosclerosis·N Blaes, C Covacho
May 1, 1986·Journal of Immunological Methods·P A LandoT S Edgington
Nov 1, 1988·Thrombosis Research·J L FrancisI Taylor
Sep 20, 1985·Biochimica Et Biophysica Acta·A Falanga, S G Gordon
Jul 15, 1997·Thrombosis Research·W P MielickiS G Gordon
Oct 31, 2003·Critical Reviews in Oncology/hematology·Mario MandalàSandro Barni
Aug 30, 2008·The New England Journal of Medicine·Bruce Furie, Barbara C Furie
Mar 1, 1986·The Journal of Experimental Medicine·P P Nawroth, D M Stern
Feb 14, 2006·Current Opinion in Pediatrics·Laszlo BajzarLesley G Mitchell
Jul 12, 2007·The Neurologist·Hideaki MatsuiFukashi Udaka
May 19, 2009·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Katarzyna Kaplinska, Wojciech P Mielicki
Aug 1, 1992·British Journal of Haematology·J WalshC L Geczy
Dec 1, 1993·British Journal of Haematology·M Amirkhosravi, J L Francis
Mar 1, 1994·Special Care in Dentistry : Official Publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry·S MeehanP F Mitchell
Oct 29, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jeffrey I ZwickerBruce Furie
Jul 16, 2014·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Ophira SalomonEli Konen
Apr 22, 2014·Cancer Metastasis Reviews·M RoselliF Guadagni
Mar 26, 2013·Critical Reviews in Oncology/hematology·Diana L HannaTed Wun
Feb 10, 1998·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·M E PasqualiniA R Eynard

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