A family of oligopeptide transporters is required for growth of Candida albicans on proteins

Molecular Microbiology
Oliver Reuss, Joachim Morschhäuser

Abstract

The human fungal pathogen Candida albicans can use proteins as the sole source of nitrogen for growth. The secretion of aspartic proteinases, which have been shown to contribute to virulence of C. albicans, allows the fungus to digest host proteins to produce peptides that must be taken up into the cell by specific transporters. To understand in more detail how C. albicans utilizes proteins as a nitrogen source, we undertook a comprehensive analysis of oligopeptide transporters encoded in the C. albicans genome. We identified eight OPT genes encoding putative oligopeptide transporters, almost all of which are represented by polymorphic alleles in strain SC5314. Expression of these genes was differentially induced when C. albicans was grown in YCB-BSA medium, which contains bovine serum albumin as the sole nitrogen source. Whereas deletion of single OPT genes in strain SC5314 did not affect its ability to utilize proteins as a nitrogen source, opt123delta triple mutants had a severe growth defect in YCB-BSA which was rescued by reintroduction of a single copy of OPT1, OPT2 or OPT3. In addition, forced expression of OPT4 or OPT5 under control of the ADH1 promoter also restored growth of an opt123delta mutant, demonstrating that a...Continue Reading

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Citations

Feb 25, 2011·The Journal of Membrane Biology·Kenny M Gomolplitinant, Milton H Saier
Mar 18, 2008·The Journal of Antimicrobial Chemotherapy·Brian G OliverTheodore C White
Oct 8, 2009·Plant & Cell Physiology·Sharon PikeWalter Gassmann
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