A family of silicon transporter structural genes in a pennate diatom Synedra ulna subsp. danica (Kütz.) Skabitsch

PloS One
A M MarchenkovAlexander A Bondar

Abstract

Silicon transporters (SIT) are the proteins, which capture silicic acid in the aquatic environment and direct it across the plasmalemma to the cytoplasm of diatoms. Diatoms utilize silicic acid to build species-specific ornamented exoskeletons and make a significant contribution to the global silica cycle, estimated at 240 ±40 Tmol a year. Recently SaSIT genes of the freshwater araphid pennate diatom Synedra acus subsp. radians are found to be present in the genome as a cluster of two structural genes (SaSIT-TD and SaSIT-TRI) encoding several concatenated copies of a SIT protein each. These structural genes could potentially be transformed into "mature" SIT proteins by means of posttranslational proteolytic cleavage. In the present study, we discovered three similar structural SuSIT genes in the genome of a closely related freshwater diatom Synedra ulna subsp. danica. Structural gene SuSIT1 is identical to structural gene SuSIT2, and the two are connected by a non-coding nucleotide DNA sequence. All the putative "mature" SITs contain conserved amino acid motifs, which are believed to be important in silicon transport. The data obtained suggest that the predicted "mature" SIT proteins may be the minimal units necessary for the t...Continue Reading

References

Feb 20, 1997·Nature·M HildebrandJ I Schroeder
Jun 17, 1998·Genes & Development·V Cryns, J Yuan
May 23, 2000·Nature·P PondavenJ L Reyss
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Emanuel Epstein
Oct 2, 2004·Science·E Virginia ArmbrustDaniel S Rokhsar
Aug 17, 2006·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·Ye V LikhoshwayM A Grachev
Mar 28, 2008·Molecular Biology and Evolution·Si Quang Le, Olivier Gascuel
Oct 17, 2008·Nature·Chris BowlerIgor V Grigoriev
Jul 20, 2012·Annual Review of Marine Science·Paul J Tréguer, Christina L De La Rocha
Feb 15, 2013·Proceedings. Biological Sciences·Alan O MarronGiselle Walker
Nov 29, 2010·Genes·P J Hastings
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
May 6, 2015·Doklady. Biochemistry and Biophysics·Y P GalachyantsM A Grachev
Dec 18, 2015·Nucleic Acids Research·Robert D FinnAlex Bateman
Feb 5, 2016·Nature Communications·Grażyna M DurakGlen L Wheeler
Jun 17, 2016·Nature Communications·Michael J KnightPaul Curnow
Oct 18, 2016·Journal of Phycology·Colleen A DurkinE Virginia Armbrust
Jun 24, 2016·Molecular Ecology·Katharine L Korunes, Mohamed A F Noor
Oct 30, 2016·Nucleic Acids Research·Supratim MukherjeeT B K Reddy
Jan 7, 2017·Doklady. Biochemistry and Biophysics·A M MarchenkovM A Grachev
Jan 17, 2017·Nature·Thomas MockIgor V Grigoriev

❮ Previous
Next ❯

Citations

Sep 29, 2019·Scientific Data·Yuri Pavlovich GalachyantsMikhail Aleksandrovich Grachev

❮ Previous
Next ❯

Datasets Mentioned

BETA
MF971079
MF971078

Methods Mentioned

BETA
PCR
electrophoresis
dissection

Software Mentioned

TD
RaxML
UniCMLD
Synedra
MEGA
SaSIT
TMPred
TRI
BioEdit
Vector NTI

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.