A FAP46 mutant provides new insights into the function and assembly of the C1d complex of the ciliary central apparatus.

Journal of Cell Science
Jason M BrownG B Witman

Abstract

Virtually all motile eukaryotic cilia and flagella have a '9+2' axoneme in which nine doublet microtubules surround two singlet microtubules. Associated with the central pair of microtubules are protein complexes that form at least seven biochemically and structurally distinct central pair projections. Analysis of mutants lacking specific projections has indicated that each may play a unique role in the control of flagellar motility. One of these is the C1d projection previously shown to contain the proteins FAP54, FAP46, FAP74 and FAP221/Pcdp1, which exhibits Ca(2+)-sensitive calmodulin binding. Here we report the isolation and characterization of a Chlamydomonas reinhardtii null mutant for FAP46. This mutant, fap46-1, lacks the C1d projection and has impaired motility, confirming the importance of this projection for normal flagellar movement. Those cells that are motile have severe defects in phototaxis and the photoshock response, underscoring a role for the C1d projection in Ca(2+)-mediated flagellar behavior. The data also reveal for the first time that the C1d projection is involved in the control of interdoublet sliding velocity. Our studies further identify a novel C1d subunit that we term C1d-87, give new insight into...Continue Reading

References

Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S M KingG B Witman
Jan 1, 1986·Methods in Enzymology·G B Witman
Dec 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·D S Gorman, R P Levine
Aug 1, 1980·The Journal of Cell Biology·M BessenG B Witman
Apr 24, 1996·Molecular & General Genetics : MGG·D R StevensS Purton
Nov 1, 1993·Trends in Cell Biology·G B Witman
Apr 21, 2005·Analytical Biochemistry·David González-BallesterEmilio Fernández
Jul 7, 2005·The Journal of Cell Biology·Gregory J PazourGeorge B Witman
Feb 14, 2007·The Journal of Cell Biology·Karl-Ferdinand Lechtreck, George B Witman
Nov 28, 2007·Molecular and Cellular Biology·Lance LeeMark D Fleming
May 9, 2009·BMC Genomics·Alan L KwanGary D Stormo
Jan 1, 2009·Methods in Cell Biology·David R Mitchell, Brandon Smith
Apr 28, 2010·The Journal of Cell Biology·Christen G DiPetrillo, Elizabeth F Smith
Jun 22, 2011·Cytoskeleton·Candice A ElamWinfield S Sale
Oct 15, 2011·Molecular Biology of the Cell·Christen G DiPetrillo, Elizabeth F Smith
Mar 1, 2012·The Journal of Cell Biology·Luis AlvarezU Benjamin Kaupp

❮ Previous
Next ❯

Citations

Apr 17, 2013·The Journal of Cell Biology·Karl F LechtreckGeorge B Witman
Oct 2, 2015·Molecular Biology of the Cell·Jovenal T San AgustinGeorge B Witman
Jan 3, 2013·Cytoskeleton·Blanca I Carbajal-GonzálezDaniela Nicastro
Aug 1, 2015·Molecular Biology of the Cell·Casey W McKenzieLance Lee
Jun 9, 2015·Current Biology : CB·Jason M BrownGeorge B Witman
Nov 5, 2016·Journal of Proteome Research·Yves VandenbrouckCharles Pineau
Oct 23, 2016·Cold Spring Harbor Perspectives in Biology·Thomas D Loreng, Elizabeth F Smith
Jun 4, 2013·Current Protocols in Cell Biology·Branch CraigeGeorge B Witman
Apr 14, 2016·Journal of Cell Science·Tomohiro KuboGeorge B Witman
Nov 2, 2019·The Journal of Cell Biology·Gang FuDaniela Nicastro
May 17, 2019·The Journal of Cell Biology·Lei ZhaoGeorge B Witman
Jun 20, 2020·Experimental Animals·Haruhiko MiyataMasahito Ikawa
Apr 4, 2021·International Journal of Molecular Sciences·Zuzanna SamselEwa Joachimiak
Oct 16, 2021·Journal of Cell Science·Kai CaiDaniela Nicastro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.