PMID: 7686260Jul 1, 1993Paper

A fast and simple method to determine the clonal relationship among human T-cell lymphocytes

Mutation Research
J G de BoerB W Glickman


For the analysis of mutation in human T-cell lymphocytes, it is crucial to determine the clonal relationship between isolated mutants, particularly if they harbour identical mutations. Here we report an efficient method to determine the clonal relationships between these cells. The method is based on the analysis of restriction fragment length polymorphisms of a polymerase chain reaction amplified, rearranged T-cell receptor gamma-gene. As few as 600 cells are sufficient, regular agarose gels can be used for the separation of the restriction fragments, no radioactive label is required, and results can be obtained in 2 days.


Jun 4, 1992·Nature·A L Goldberg, K L Rock
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A BourguinJ Sklar
Apr 14, 1983·Nature·S Tonegawa

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Oct 12, 2000·Mutation Research·M KubotaY Wakazono
Jan 1, 1996·Environmental and Molecular Mutagenesis·L S Clark, J A Nicklas
Jan 1, 1997·Environmental and Molecular Mutagenesis·A D da Cruz, B W Glickman
Feb 24, 2004·Genes, Chromosomes & Cancer·Ying-Wei LinPeter D Aplan
Jan 1, 1995·Environmental and Molecular Mutagenesis·J G de Boer

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