A fast, low-cost and efficient method for the diagnosis of sperm DNA fragmentation in several species

Reproduction in Domestic Animals = Zuchthygiene
B R RuiRjg Pereira

Abstract

Sperm DNA fragmentation is a condition that interferes directly in the reproductive efficiency. Currently, there are several methods for assessing the sperm DNA integrity, such as Alkaline Comet, TUNEL and Sperm Chromatin Structure Assay. However, many of these techniques are laborious and require high-precision equipment. Thus, the development of new techniques can optimize the evaluation of sperm DNA damage. Therefore, the aim of this study was to standardize the toluidine blue (TB) stain technique for the analysis of DNA fragmentation of dog, cat, bull, stallion and ram spermatozoa. For this purpose, we used six animals of each specie (n = 30), in reproductive age. Sperm was collected by different methods according to the particularities of each species, and such samples were divided into two aliquots: a sperm sample was kept at 5°C (considered as intact sperm DNA), and the remaining samples were submitted to the induction of DNA fragmentation by exposure to ultraviolet light for 4 hr. Samples were then mixed with the intact sample to obtain known and progressive proportions of sperm with fragmented DNA (0%, 25%, 50%, 75% and 100%). Semen smears were performed and subjected to staining with TB. Blue-stained spermatozoa were ...Continue Reading

References

Jul 27, 2001·Molecular Reproduction and Development·S Chatterjee, C Gagnon
Feb 22, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jekaterina ErenpreisaDace Pjanova
May 2, 2003·Human Reproduction·Mehdi BenchaibJean François Guérin
Jun 27, 2003·Journal of Andrology·Julie BaumberStuart A Meyers
Sep 12, 2007·Reproduction in Domestic Animals = Zuchthygiene·E C C CeleghiniC F Raphael
Dec 9, 2008·Journal of Andrology·Armand Zini, Mark Sigman
May 8, 2010·Human Reproduction·Luke SimonSheena E M Lewis
Sep 10, 2011·Journal of Assisted Reproduction and Genetics·Monis Bilal ShamsiRima Dada
Mar 14, 2013·PloS One·Robert HermesThomas B Hildebrandt
Aug 14, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Renata SimõesMayra Elena Ortiz Assumpção
Aug 21, 2013·Reproductive Biomedicine Online·Sheena E M LewisParviz Gharagozloo
Aug 16, 2015·Animal Reproduction Science·L KrakowskiA Chałabis-Mazurek
Oct 25, 2016·Reproduction in Domestic Animals = Zuchthygiene·R B FloresC I Vannucchi

❮ Previous
Next ❯

Citations

May 17, 2019·Journal of Assisted Reproduction and Genetics·Koray Yildiz, Sengul Yuksel
Oct 12, 2019·Zygote : the Biology of Gametes and Early Embryos·Thais Rose Dos Santos Hamilton, Mayra Elena Ortiz D'Ávila Assumpção
Jul 23, 2020·Basic and Clinical Andrology·Daniel S R AngrimaniCamila I Vannucchi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.