A feedback mechanism converts individual cell features into a supracellular ECM structure in Drosophila trachea

ELife
Arzu Öztürk-ÇolakJordi Casanova

Abstract

The extracellular matrix (ECM), a structure contributed to and commonly shared by many cells in an organism, plays an active role during morphogenesis. Here, we used the Drosophila tracheal system to study the complex relationship between the ECM and epithelial cells during development. We show that there is an active feedback mechanism between the apical ECM (aECM) and the apical F-actin in tracheal cells. Furthermore, we reveal that cell-cell junctions are key players in this aECM patterning and organisation and that individual cells contribute autonomously to their aECM. Strikingly, changes in the aECM influence the levels of phosphorylated Src42A (pSrc) at cell junctions. Therefore, we propose that Src42A phosphorylation levels provide a link for the ECM environment to ensure proper cytoskeletal organisation.

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Citations

Jun 22, 2017·Insect Biochemistry and Molecular Biology·Gyöngyi CinegeÉva Kurucz
Jan 18, 2017·Development, Growth & Differentiation·Shigeo Hayashi, Bo Dong
Jul 6, 2017·PLoS Genetics·Ivette Olivares-Castiñeira, Marta Llimargas
Mar 2, 2017·Cold Spring Harbor Perspectives in Biology·Ilenia BernasconeFernando Martin-Belmonte
Jul 28, 2019·Nature Communications·Dong-Yuan ChenDavid Bilder
Aug 8, 2018·Genetics·Arzu Öztürk-ÇolakSofia J Araújo
May 31, 2018·Genetics·Shigeo Hayashi, Takefumi Kondo
Aug 10, 2020·Scientific Reports·Matthias Behr, Dietmar Riedel
Feb 5, 2021·Current Opinion in Genetics & Development·Victoria J Lloyd, Nicola J Nadeau
Jun 24, 2021·The Journal of Cell Biology·Paolo RonchiYannick Schwab

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Methods Mentioned

BETA
transmission electron microscopy
transgenic
light microscopy

Software Mentioned

Fiji
Photoshop

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