A ferredoxin-type iron-sulfur protein gene, frx B, is expressed in the chloroplasts of tobacco and spinach

Plant Molecular Biology
C H Lin, M Wu

Abstract

Previously, a ferredoxin-type iron-sulfur protein, frx B protein, was identified in a high-salt extract of the purified thylakoid membrane of Chlamydomonas reinhardtii, a unicellular green alga. Polyclonal antibody was raised against a synthetic pentadecameric peptide with an amino acid sequence corresponding to the highly conserved region of the putative frx B proteins of 3 land plants. In this report, protein(s) reacting strongly and specifically with this antibody was detected in the equivalent high-salt extract prepared from purified chloroplast of spinach and tobacco. One strong reaction polypeptide band from tobacco chloroplast was purified from SDS-polyacrylamide gel and subjected to endoproteinase lys C digestion. The resulting polypeptides were separated by reversed-phase chromatography. N-terminal sequencing of 3 purified polypeptides revealed that the protein is encoded by the 'frxB gene' identified from DNA sequence analysis.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·N H Chua, P Bennoun
Jan 11, 1988·Nucleic Acids Research·P P Dunn, J C Gray
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P Bennoun

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Citations

Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·T WakasugiM Sugiura

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