A fetal human heart cardiac-inducing RNA (CIR) promotes the differentiation of stem cells into cardiomyocytes

In Vitro Cellular & Developmental Biology. Animal
Andrei KochegarovL F Lemanski

Abstract

A specific human fetal heart RNA has been discovered, which has the ability to induce myocardial cell formation from mouse embryonic and human-induced pluripotent stem cells in culture. In this study, commercially obtained RNA from human fetal heart was cloned, sequenced, and synthesized using standard laboratory approaches. Molecular analyses of the specific fetal cardiac-inducing RNA (CIR), revealed that it is a fragment of N-sulfoglucosaminesulfohydrolase and the caspase recruitment domain family member 14 precursor. Stem cells transfected with CIRs often form into spindle-shaped cells characteristic of cardiomyocytes,and express the cardiac-specific contractile protein marker, troponin-T, in addition to tropomyosin and α-actinin as detected by immunohistochemical staining. Expression of these contractile proteins showed organization into sarcomeric myofibrils characteristic of striated cardiac muscle cells. Computer analyses of the RNA secondary structures of the active CIR show significant similarities to a RNA from salamander or myofibril-inducing RNA (MIR), which also promotes non-muscle cells to differentiate into cardiac muscle. Thus, these two RNAs, salamander MIR and the newly discovered human-cloned CIR reported her...Continue Reading

References

Dec 24, 1996·Biochemical and Biophysical Research Communications·L F LemanskiD K Dube
Apr 23, 2002·Nature Genetics·Amy J SehnertDidier Y R Stainier
May 30, 2002·Journal of Anatomy·C MummeryM Pera
Apr 6, 2007·Biochemical and Biophysical Research Communications·Gian Franco SferrazzaLarry F Lemanski
Nov 20, 2014·In Vitro Cellular & Developmental Biology. Animal·Ashley Moses-ArmsLarry F Lemanski

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Citations

Jul 26, 2012·PloS One·Charlotte Rouzaud-LabordeNathalie Pizzinat

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