A fibrinogen-related protein (Mnfico3) acts as a novel pattern recognition receptor in Macrobrachium nipponense

Fish & Shellfish Immunology
Nan QinFengsong Liu

Abstract

Fibrinogen-related proteins (FREPs) are widely found in both vertebrates as well as invertebrates, and they play a crucial role in host immunity. In this study, we isolated a novel ficolin gene (Mnfico3) from the oriental river prawn Macrobrachium nipponense. The complete cDNA sequence of Mnfico3 was 1133 bp long, containing an open reading frame of 765 bp coding for Mnfico3, a protein consisting of 254 amino acids. The Mnfico3 protein contained a putative N-terminal signal peptide and a fibrinogen-related protein domain present at the C-terminal. Phylogenetic analysis indicated that Mnfico3 had a closer evolutionary relationship with vertebrate ficolins than with its invertebrate homologues. Tissue distribution analysis indicated that Mnfico3 was predominantly expressed in muscle, in which its transcription was increased following bacterial challenge by Aeromonas veronii. Function analysis using recombinant protein revealed that rMnFico3 had broad-spectrum binding capacity to a variety of microorganisms and pathogen-associated molecular pattern (PAMP) ligands. Furthermore, rMnFico3 exhibited Ca2+-dependent agglutinating activity against microbes in vitro, and ability to attach to the hemocyte surface which promoted phagocytosi...Continue Reading

References

Oct 20, 1998·Immunobiology·J Lu, Y Le
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 28, 2002·Annual Review of Immunology·Charles A Janeway, Ruslan Medzhitov
Oct 25, 2002·Immunobiology·Misao Matsushita, Teizo Fujita
Apr 25, 2009·Cellular Microbiology·Thomas Areschoug, Siamon Gordon
May 19, 2009·Developmental and Comparative Immunology·Xian-Wei WangJin-Xing Wang
May 29, 2009·Innate Immunity·Lorena VazquezEdgar Zenteno
Apr 9, 2010·Journal of Innate Immunity·Misao Matsushita
Apr 17, 2010·Marine Biotechnology·Ikuo HironoTakashi Aoki
Jun 22, 2010·Fish & Shellfish Immunology·Pacharaporn AngthongPikul Jiravanichpaisal
Nov 11, 2010·Journal of Innate Immunity·Patrick C Hanington, Si-Ming Zhang
Jan 28, 2014·Developmental and Comparative Immunology·Xiao-Wen ZhangQian Ren
Nov 6, 2015·Results and Problems in Cell Differentiation·Coen M Adema
Oct 23, 2016·Fish & Shellfish Immunology·Fujun HouXiaolin Liu
May 11, 2019·International Journal of Biological Macromolecules·Nan QinFengsong Liu

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Laboratory and Clinical Medicine
M S RabaaO D Ratnoff
Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
Ping WuSongnian Hu
Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control
Qin LiuXiao-Nong Zhou
Parasites & Vectors
Jan SterbaLibor Grubhoffer
© 2021 Meta ULC. All rights reserved