A fibrous-bed bioreactor for continuous production of monoclonal antibody by hybridoma

Advances in Biochemical Engineering/biotechnology
Shang-Tian YangChunnuan Chen

Abstract

A fibrous-bed bioreactor (FBB) has been developed to culture hybridoma cells for long-term continuous production of monoclonal antibody (MAb). A non-woven polyester fibrous matrix was used to immobilize the cells to reach a high viable cell density of 3 x 10(8) cells cm(-3) packed bed, which gave a high volumetric MAb productivity of 1 g L(-1) day(-1) under continuous feed conditions with the medium containing 10% serum. Reducing the medium serum content to 1% increased MAb production to 6.5 g L(-1) day(-1) in a repeated batch FBB culture. MAb production was higher at higher dissolved oxygen (DO) levels in the range between 10% and 70% of air saturation, although DO did not significantly affect glucose metabolism and lactate production. The medium LDH (lactate dehydrogenase) level increased dramatically when the DO level was decreased from 30% to 10%, suggesting that a critical DO level of approximately 30% is necessary for maintaining the FBB culture for long-term operation. Compared with suspension cultures in T-flasks and spinner flasks, the FBB culture had a lower lactate yield from glucose (0.80 vs. 0.91 g g(-1), produced MAb at a higher concentration (up to 442 mg L(-1) vs. 83.5 mg L(-1), and was stable for continuous lon...Continue Reading

Citations

Oct 7, 2009·Biotechnology Progress·Christopher J DetzelBernard J van Wie
Jul 26, 2006·Biotechnology and Bioengineering·Anli Ouyang, Shang-Tian Yang
Jan 1, 2010·Biotechnology Progress·Maria Elisa RodriguesRosário Oliveira
Sep 27, 2013·Applied Biochemistry and Biotechnology·Duygu Ayyildiz-TamisSaime Ismet Deliloglu-Gurhan

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