A filovirus-unique region of Ebola virus nucleoprotein confers aberrant migration and mediates its incorporation into virions.

Journal of Virology
Wei ShiGary J Nabel

Abstract

The Ebola virus nucleoprotein (NP) is an essential component of the nucleocapsid, required for filovirus particle formation and replication. Together with virion protein 35 (VP35) and VP24, this gene product gives rise to the filamentous nucleocapsid within transfected cells. Ebola virus NP migrates aberrantly, with an apparent molecular mass of 115 kDa, although it is predicted to encode an approximately 85-kDa protein. In this report, we show that two domains of this protein determine this aberrant migration and that this region mediates its incorporation into virions. These regions, amino acids 439 to 492 and amino acids 589 to 739, alter the mobility of Ebola virus NP by sodium dodecyl sulfate-polyacrylamide gel electrophoresis by 5 and 15 kDa, respectively, and confer similar effects on a heterologous protein, LacZ, in a position-independent fashion. Furthermore, when coexpressed with VP40, VP35, and VP24, this region mediated incorporation of NP into released viruslike particles. When fused to chimeric paramyxovirus NPs derived from measles or respiratory syncytial virus, this domain directed these proteins into the viruslike particle. The COOH-terminal NP domain comprises a conserved highly acidic region of NP with predi...Continue Reading

References

Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Apr 1, 1994·The Journal of General Virology·S BeckerH Feldmann
Apr 1, 1996·Journal of Virology·H FeldmannH J Schnittler
Dec 1, 1995·Virus Research·T W Geisbert, P B Jahrling
Jun 27, 2000·Bioinformatics·L J McGuffinD T Jones
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·R N HartyF P Hayes
Mar 29, 2001·Protein Science : a Publication of the Protein Society·H Neurath
Apr 21, 2001·Virology·J TimminsW Weissenhorn
May 3, 2001·Journal of Virology·L D JasenoskyY Kawaoka
Jun 23, 2001·Protein Science : a Publication of the Protein Society·L M IakouchevaE J Ackerman
Mar 6, 2002·The Journal of Experimental Medicine·Sina BavariM Javad Aman
Apr 23, 2002·Journal of Virology·Takeshi NodaYoshihiro Kawaoka
May 8, 2003·The Journal of Biological Chemistry·Nyet Kui WongManish S Patankar
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·H Jane Dyson, Peter E Wright
Mar 31, 2006·Journal of Virology·Shinji WatanabeYoshihiro Kawaoka
Aug 4, 2006·PLoS Computational Biology·Chad HaynesLilia M Iakoucheva
Dec 7, 2007·Trends in Biochemical Sciences·Peter Tompa, Monika Fuxreiter

❮ Previous
Next ❯

Citations

Sep 10, 2013·Trends in Microbiology·Tim F BoothDaniel R Beniac
Jan 19, 2010·Nature Structural & Molecular Biology·Daisy W LeungGaya K Amarasinghe
Mar 2, 2012·Science Translational Medicine·Mayra GarcíaGary J Nabel
Jun 24, 2010·BMC Evolutionary Biology·Derek J TaylorJeremy Bruenn
Aug 24, 2010·Future Virology·Yuliang Liu, Ronald N Harty
Nov 10, 2011·Viruses·Masayuki Horie, Keizo Tomonaga
Aug 1, 2015·FEBS Letters·Rashmi SharmaMonika Fuxreiter
Apr 20, 2010·Journal of Molecular Biology·Daisy W LeungGaya K Amarasinghe
Oct 17, 2012·Journal of Virological Methods·Jérémy PeyrolFrançoise Rollin-Genetet
Sep 23, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ji-Xia RenYong Xie
Sep 14, 2016·Biomédica : revista del Instituto Nacional de Salud·Ángela Patricia GuerraJacqueline Chaparro-Olaya
Sep 20, 2011·Molecular BioSystems·Monika Fuxreiter
Feb 20, 2016·Acta Crystallographica. Section D, Structural Biology·Laura E BakerZygmunt S Derewenda
Sep 27, 2013·Biochemistry·Jennifer M BinningGaya K Amarasinghe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.