A fine-tuning mechanism underlying self-control for autophagy: deSUMOylation of BECN1 by SENP3.

Autophagy
Kejia LiuJing Yi

Abstract

The roles of SUMOylation and the related enzymes in autophagic regulation are unclear. Based on our previous studies that identified the SUMO2/3-specific peptidase SENP3 as an oxidative stress-responsive molecule, we investigated the correlation between SUMOylation and macroautophagy/autophagy. We found that Senp3± mice showed increased autophagy in the liver under basal and fasting conditions, compared to Senp3+/+ mice. We constructed a liver-specific senp3 knockout mouse; these Senp3-deficient liver tissues showed increased autophagy as well. Autophagic flux was accelerated in hepatic and other cell lines following knockdown of SENP3, both before and after the cells underwent starvation in the form of the serum and amino acid deprivation. We demonstrated that BECN1/beclin 1, the core molecule of the BECN1-PIK3C3 complex, could be SUMO3-conjugated by PIAS3 predominantly at K380 and deSUMOylated by SENP3. The basal SUMOylation of BECN1 was increased upon cellular starvation, which enhanced autophagosome formation by facilitating BECN1 interaction with other complex components UVRAG, PIK3C3 and ATG14, thus promoting PIK3C3 activity. In contrast, SENP3 deSUMOylated BECN1, which impaired BECN1-PIK3C3 complex formation or stability...Continue Reading

References

Jul 8, 1999·Molecular Cell·T G KutateladzeM Overduin
Mar 26, 2002·FEBS Letters·Harald StenmarkPaul C Driscoll
Apr 8, 2004·Developmental Cell·Beth Levine, Daniel J Klionsky
Sep 24, 2005·Cell·Sophie PattingreBeth Levine
Oct 26, 2005·Cell Death and Differentiation·T Yorimitsu, D J Klionsky
Jun 27, 2006·Nature Cell Biology·Chengyu LiangJae U Jung
Jul 6, 2007·Autophagy·Noboru Mizushima, Tamotsu Yoshimori
Sep 25, 2007·Nature Cell Biology·Yoshinori TakahashiHong-Gang Wang
Jan 15, 2008·Cell·Beth Levine, Guido Kroemer
Jan 25, 2008·The Biochemical Journal·Jonathan M Backer
Feb 29, 2008·Nature·Noboru MizushimaDaniel J Klionsky
Oct 3, 2008·Antioxidants & Redox Signaling·Meghan B AzadSpencer B Gibson
Dec 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Qiming SunQing Zhong
Mar 10, 2009·Nature Cell Biology·Kohichi MatsunagaTamotsu Yoshimori
May 2, 2009·Cell Death and Differentiation·Y ChenS B Gibson
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Angela AlexanderCheryl Lyn Walker
Jun 2, 2010·Molecular Cell·Tsuyoshi FuruyaJunying Yuan
Aug 24, 2010·Trends in Biochemical Sciences·Ruth Scherz-Shouval, Zvulun Elazar
Oct 6, 2010·The Journal of Cell Biology·Sabrina Di BartolomeoGian Maria Fimia
Feb 1, 2011·Trends in Cell Biology·David G McEwan, Ivan Dikic
Feb 12, 2011·Cell Death and Differentiation·R KangD Tang
Sep 14, 2011·Cell Research·Ellen WirawanPeter Vandenabeele
Sep 23, 2011·The Biochemical Journal·Harald W PlattaHarald Stenmark
Nov 15, 2011·Cell·Noboru Mizushima, Masaaki Komatsu
Dec 16, 2011·Autophagy·Ellen WirawanPeter Vandenabeele
Mar 14, 2012·Cell Research·Daniel J Klionsky, James H Hurley
Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yonghua YangKapil N Bhalla
May 21, 2013·Nature Cell Biology·Ryan C RussellKun-Liang Guan
Aug 27, 2013·The EMBO Journal·Pengyan XiaZusen Fan
Aug 27, 2014·Seminars in Cell & Developmental Biology·E A Dunlop, A R Tee
Sep 27, 2014·Cell Death and Differentiation·G FilomeniF Cecconi
Nov 5, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Willayat Yousuf WaniJianhua Zhang

❮ Previous
Next ❯

Citations

Oct 15, 2020·Animals : an Open Access Journal From MDPI·Wen GaoBichun Li
Jul 14, 2021·Open Biology·Zenghua ShengShufang Liang
Aug 7, 2021·Biomolecules·Iman F Fergani, Luciana R Frick

❮ Previous
Next ❯

Methods Mentioned

BETA
acetylation
electron microscopy
co-IP
co-immunoprecipitation
pull-down
ubiquitination
biosensor
transfection
FRET
lipidation

Software Mentioned

ImageQuant
GraphPad Prism
ImageJ
SUMOplot TM

Related Concepts

Related Feeds

ATG proteins

The discovery of autophagy-related ('ATG') proteins in the 1990s greatly advanced the mechanistic understanding of autophagy and clarified the fact that autophagy serves important roles in various biological processes.

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Autophagy & Aging: Inhibitors

The feed focuses on the role of nuclear export inhibitors and their effect on autophagy and the aging process.

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.