A Fixed Cohort Field Study of Gene Expression in Circulating Leukocytes From Dairy Cows With and Without Mastitis

Frontiers in Veterinary Science
Craig S McConnelAmber Adams-Progar

Abstract

Specifically designed gene expression studies can be used to prioritize candidate genes and identify novel biomarkers affecting resilience against mastitis and other diseases in dairy cattle. The primary goal of this study was to assess whether specific peripheral leukocyte genes expressed differentially in a previous study of dairy cattle with postpartum disease, also would be expressed differentially in peripheral leukocytes from a diverse set of different dairy cattle with moderate to severe clinical mastitis. Four genes were selected for this study due to their differential expression in a previous transcriptomic analysis of circulating leukocytes from dairy cows with and without evidence of early postpartum disease. An additional 15 genes were included based on their cellular, immunologic, and inflammatory functions associated with resistance and tolerance to mastitis. This fixed cohort study was conducted on a conventional dairy in Washington state. Cows >50 days in milk (DIM) with mastitis (n = 12) were enrolled along with healthy cows (n = 8) selected to match the DIM and lactation numbers of mastitic cows. Blood was collected for a complete blood count (CBC), serum biochemistry, leukocyte isolation, and RNA extraction ...Continue Reading

References

Nov 4, 2000·Biochimica Et Biophysica Acta·D R FlowerC E Sansom
Jan 10, 2003·The New England Journal of Medicine·Craig Gerard
Sep 10, 2003·Journal of Leukocyte Biology·Niels C RiedemannPeter A Ward
Oct 15, 2003·Veterinary Research·Henri SeegersFrançois Beaudeau
Dec 17, 2003·Shock·Ren-Feng GuoPeter A Ward
Jan 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·C Chace TydellMichael E Selsted
Feb 2, 2006·BMC Molecular Biology·Andreas SchroederThomas Ragg
Nov 1, 2006·Veterinary Immunology and Immunopathology·T L RobinsonJ Sutherland
Jan 19, 2007·Journal of the American Society of Nephrology : JASN·Kai M Schmidt-OttJonathan Barasch
Feb 21, 2007·Cellular and Molecular Life Sciences : CMLS·N Mookherjee, R E W Hancock
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sangwoo ChoRoy A Mariuzza
Jul 31, 2007·Veterinary Immunology and Immunopathology·Wenjing Tao, Bonnie Mallard
May 16, 2008·Journal of Leukocyte Biology·Sailasree NemaliMark T Quinn
Oct 18, 2008·Nature Reviews. Immunology·David S Schneider, Janelle S Ayres
Nov 3, 2010·Veterinary Immunology and Immunopathology·Manuela RinaldiAnthony V Capuco
Dec 25, 2010·Journal of Dairy Science·M G H StevensC Burvenich
Jun 28, 2011·Journal of Dairy Science·C Pinzón-Sánchez, P L Ruegg
Aug 30, 2011·Briefings in Bioinformatics·Pascale GaudetPaul D Thomas
Oct 15, 2011·Journal of Mammary Gland Biology and Neoplasia·Gina M Pighetti, A A Elliott
Mar 1, 2012·Science·Ruslan MedzhitovMiguel P Soares
Jan 3, 2013·Arthritis and Rheumatism·Rangaiah ShashidharamurthyMatam Vijay-Kumar
Nov 16, 2013·Nature Chemical Biology·Ashley L HilchieRobert E W Hancock
May 20, 2018·Veterinary Immunology and Immunopathology·John J BromfieldSossi M Iacovides
Oct 31, 2018·Proceedings of the National Academy of Sciences of the United States of America·Marten SchefferMarcel G M Olde Rikkert
Nov 6, 2018·Nucleic Acids Research·UNKNOWN UniProt Consortium
Jan 24, 2019·Annual Review of Immunology·Rui MartinsMiguel P Soares
Jun 28, 2019·Frontiers in Immunology·Younghee LeeSeon Hee Chang

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BETA
ELISA
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Software Mentioned

nSolver
nCounter
nCounter Advanced Analysis
R R Project for Statistical Computing
poLCA
Nanostring nCounter Analysis System
nSolver Analysis

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