A flavonoid inhibitor of 5-lipoxygenase inhibits leukotriene production following ischemia in gerbil brain

Stroke; a Journal of Cerebral Circulation
M BanL Levine


Leukotrienes C4 and D4 are arachidonic acid metabolites that constrict blood vessels and enhance vascular permeability; their biosynthesis is initiated by the reaction of arachidonic acid with 5-lipoxygenase enzyme. After bilateral carotid artery occlusion for 15 minutes and reperfusion of the gerbil brain for 15 minutes, we determined the brain tissue concentrations of leukotrienes C4 and D4 by radioimmunoassay; they had increased from a baseline concentration of less than 1 to a mean +/- SEM concentration of 12.8 +/- 3.9 pmol/g brain. We also studied the effect of a flavonoid 5-lipoxygenase inhibitor on leukotriene production in the reperfused gerbil brain. A water-soluble flavonoid (5-hexyloxy-3',4'-dihydroxy-6,7-dimethoxyflavone 4'-disodium phosphate) was administered intravenously at a dose of 200 mg/kg body wt; 15 minutes later, both carotid arteries were occluded. The enhanced production of leukotrienes C4 and D4 in the reperfused brain was reduced by approximately 80% (from a mean +/- SEM of 12.8 +/- 3.9 to 2.2 +/- 1.3 pmol/g brain) in the presence of the 5-lipoxygenase inhibitor. The flavonoid did not affect the production of prostaglandin D2, the concentration of which also increased in the reperfused ischemic brain.


Dec 1, 1975·Japanese Journal of Pharmacology·H NagaiA Koda
Feb 14, 1979·Biochemical and Biophysical Research Communications·R J Gaudet, L Levine
Oct 31, 1983·Biochemical and Biophysical Research Communications·T YoshimotoS Watanabe-Kohno
Sep 1, 1984·The Journal of Allergy and Clinical Immunology·S YamamotoS Watanabe-Kohno
Jan 1, 1983·Prostaglandins, Leukotrienes, and Medicine·O P GulatiN Gulati
Jul 1, 1983·Prostaglandins, Leukotrienes, and Medicine·P TagariD J Boullin
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J M DrazenE J Corey
May 1, 1982·Journal of Neurochemistry·H A CrockardP T Lascelles


Nov 1, 1994·Free Radical Biology & Medicine·D TrueloveJ Kalra
Jul 1, 1996·Critical Reviews in Oncology/hematology·C Denzlinger
Feb 14, 2016·Brain Research·Tamil Selvan AnthonymuthuHülya Bayır
Apr 1, 1997·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·R L Martin
Jan 1, 1991·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S AktanS Ercan
Aug 1, 1990·Prostaglandins, Leukotrienes, and Essential Fatty Acids·N ShiratsukiS Yamamoto
Aug 1, 1991·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S AktanS Ercan
Mar 23, 2004·Brain Research·Kazuo KitagawaMasatsugu Hori
Jul 5, 1991·Brain Research·N HynesF Coceani
Oct 16, 2014·Biochimica Et Biophysica Acta·Hartmut KuhnKlaus van Leyen
Jan 9, 2015·Inflammation·Adem Bozkurt ArasMurat Cosar
Oct 12, 2012·Molecular Biology Reports·Gloria Gutiérrez-Venegas, Anabel Contreras-Sánchez

Related Concepts

5-hexyloxy-3',4'-dihydroxy-6,7-dimethoxyflavone 4'-phosphate
Arachidonate Lipoxygenases
Brain Ischemia
Prostaglandin D2

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