A flexible, efficient, checkerboard immunoblot system for the detection and semiquantitation of specific antinuclear antibodies

Journal of Clinical Laboratory Analysis
R F RitchieT B Ledue


In this paper an uncomplicated method for the simultaneous detection and semiquantitation of 11 of the 12 commonly studied antinuclear antibodies (ANA) in a single run is described. This new application of checkerboard immunoblotting (CBIB) is based upon available technology and employs purified antigens which can be either purchased or produced in-house. CBIB requires no electronic instrument, can be formatted to meet the needs of the user, is rapidly performed, and has acceptable labor and materials costs. Data on the use of the method to examine available reference antisera is presented. CBIB has also proven practical for the clinical study of 18 sera, at two dilutions per membrane, for each set of specific antinuclear antibodies, also at two or more dilutions.


Jan 1, 1976·Scandinavian Journal of Immunology·E M TanP Robitaille
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 1, 1975·Annals of Internal Medicine·D D NotmanE M Tan
Feb 1, 1992·American Journal of Clinical Pathology·M Y ElkhalifaM D Poulik
Apr 1, 1992·Arthritis and Rheumatism·G NeuerF A Bautz
Feb 1, 1992·Arthritis and Rheumatism·A B MongeyE V Hess
Jan 11, 1991·Journal of Clinical Laboratory Analysis·N M MoncéN N Cappel
Apr 1, 1991·The American Journal of Medicine·M L OlsenN B Warner
Apr 11, 1991·Arthritis and Rheumatism·R D AltmanB A Michel
Oct 11, 1991·Arthritis and Rheumatism·H DangC A Feghali
Jun 1, 1991·Arthritis and Rheumatism·J EvansJ Craft
Jun 1, 1991·Arthritis and Rheumatism·I García-de la TorreL Hernández-Vazquez
Aug 1, 1990·Arthritis and Rheumatism·J W TervaertG K van der Hem
May 1, 1987·Reviews of Infectious Diseases·R A FinkelsteinD S Ludwig
May 12, 1989·Journal of Immunological Methods·D I Stott
Apr 1, 1989·Clinical Immunology and Immunopathology·F KonikoffY Shoenfeld
Apr 1, 1989·The Journal of Clinical Investigation·M D RaderM Reichlin
Feb 27, 1988·Lancet·J PritchardC Dicks-Mireaux
Feb 27, 1988·Lancet·M MäkiJ K Visakorpi
Jun 2, 1988·The New England Journal of Medicine·M C TotoritisR L Rubin
Feb 10, 1988·Journal of Immunological Methods·L StitzR M Zinkernagel
May 1, 1988·The Journal of Experimental Medicine·E Ben-ChetritE M Tan
Jun 1, 1985·Clinical Immunology and Immunopathology·R A EisenbergP L Cohen
Sep 1, 1985·The Journal of Clinical Investigation·V M Holers, B L Kotzin
Sep 16, 1981·Biochemical and Biophysical Research Communications·P J White, S O Hoch
May 1, 1982·Journal of Clinical Pathology·R P StokesR A Thompson


Dec 2, 1994·Journal of Immunological Methods·T B LedueR F Ritchie
Feb 8, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·R F Ritchie
Oct 13, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·W Y CraigR F Ritchie
Jan 1, 1993·Journal of Clinical Laboratory Analysis·W Y CraigR F Ritchie

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