A flexible template boundary element in the RNA subunit of fission yeast telomerase

The Journal of Biological Chemistry
Jessica A BoxPeter Baumann


Telomerase adds telomeric repeat sequences to chromosome ends using a short region of its RNA subunit as a template. Telomerase RNA subunits are phylogenetically highly divergent, and different strategies have evolved to demarcate the boundary of the template region. The recent identification of the gene encoding telomerase RNA in the fission yeast Schizosaccharomyces pombe (ter1+) has opened the door for structure-function analyses in a model that shares many features with the telomere maintenance machinery of higher eukaryotes. Here we describe a structural element in TER1 that defines the 5' boundary of the template. Disruption of a predicted long range base pairing interaction between template-adjacent nucleotides and a sequence further upstream resulted in reverse transcription beyond the template region and caused telomere shortening. Normal telomere length was restored by combining complementary nucleotide substitutions in both elements, showing that base pairing, not a specific sequence, limits reverse transcription beyond the template. The template boundary described here resembles that of budding yeasts and some mammalian telomerases. However, unlike any previously characterized boundary element, part of the paired re...Continue Reading


Oct 18, 1991·Cell·D P Romero, Elizabeth H Blackburn
Dec 8, 1995·Science·Virginia A Zakian
Oct 21, 1994·Science·M S Singer, D E Gottschling
Sep 15, 1995·Genes & Development·C Autexier, Carol W Greider
Aug 3, 1995·Nature·M J McEachern, Elizabeth H Blackburn
Apr 11, 1995·Nucleic Acids Research·M McCormick-Graham, D P Romero
Feb 1, 1997·Trends in Biochemical Sciences·P König, D Rhodes
Apr 1, 1997·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Raymund J Wellinger, D Sen
May 23, 1998·Current Genetics·M CohnElizabeth H Blackburn
May 19, 1998·Trends in Biochemical Sciences·Y HiraokaElizabeth H Blackburn
Mar 18, 2000·Cell·J L ChenCarol W Greider
May 8, 2000·Science·Yehuda TzfatiElizabeth H Blackburn
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·C H HaeringThomas R Cech
Jun 29, 2000·Annual Review of Biochemistry·Kathleen Collins
Oct 12, 2000·Molecular Biology of the Cell·Peter Baumann, Thomas R Cech
Oct 5, 2001·Molecular and Cellular Biology·K Förstemann, J Lingner
Feb 19, 2002·Genes & Development·Cary K LaiKathleen Collins
Jul 2, 2002·Lancet·Tom VulliamyPhilip J Mason
Nov 25, 2003·Genes & Development·Jiunn-Liang Chen, Carol W Greider
Jan 28, 2004·Cell·Thomas R Cech
Apr 15, 2004·Trends in Biochemical Sciences·Jiunn-Liang Chen, Carol W Greider
Jul 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·David C Zappulla, Thomas R Cech
Jul 10, 2004·Current Biology : CB·Alain T DandjinouRaymund J Wellinger
Sep 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jue LinElizabeth H Blackburn
Apr 9, 2005·The New England Journal of Medicine·Hiroki YamaguchiNeal S Young
Jun 21, 2005·Molecular and Cellular Biology·Jeremy T BunchPeter Baumann
Oct 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mary ArmaniosCarol W Greider
May 23, 2006·Current Opinion in Structural Biology·Carla A Theimer, Juli Feigon
Jul 11, 2006·Nature Reviews. Molecular Cell Biology·Kathleen Collins
Dec 25, 2007·Nature Structural & Molecular Biology·Christopher J Webb, Virginia A Zakian
Dec 25, 2007·Nature Structural & Molecular Biology·Jessica LeonardiPeter Baumann
Jan 8, 2008·Nature Structural & Molecular Biology·Manuel Ares, Kausik Chakrabarti


Jan 10, 2013·Nature Reviews. Molecular Cell Biology·Jayakrishnan Nandakumar, Thomas R Cech
Oct 25, 2012·Nucleic Acids Research·Xiaodong QiJulian J-L Chen
Jul 28, 2010·Cold Spring Harbor Perspectives in Biology·Elizabeth H Blackburn, Kathleen Collins
Oct 31, 2012·Genes & Development·Catherine Cifuentes-RojasDorothy E Shippen
Nov 20, 2012·Biochemistry. Biokhimii︠a︡·E M SmekalovaO A Dontsova
Aug 10, 2012·RNA·Emily D Egan, Kathleen Collins
Dec 25, 2015·Cell Cycle·Christopher J Webb, Virginia A Zakian
Aug 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Christopher J Webb, Virginia A Zakian
Jan 21, 2016·Scientific Reports·Rachel O NiedererDavid C Zappulla
Aug 12, 2009·Biochimica Et Biophysica Acta·Vijay G SekaranMichael B Jarstfer
Jun 12, 2013·Wiley Interdisciplinary Reviews. RNA·Natoya PeartEric J Wagner
Oct 17, 2013·The EMBO Journal·Melissa A MeffordDavid C Zappulla
Jan 20, 2015·Nature Communications·Ram KannanPeter Baumann
Jun 2, 2016·RNA Biology·Christopher J Webb, Virginia A Zakian
Jul 1, 2016·RNA Biology·Joshua D Podlevsky, Julian J-L Chen
Jul 6, 2016·Nucleic Acids Research·Joshua D PodlevskyJulian J-L Chen
Oct 8, 2016·FEBS Letters·Henrietta W BennettMegan C King
Feb 15, 2017·Leukemia Research·Alessandro AllegraCaterina Musolino
Dec 5, 2008·Nature·Jessica A BoxPeter Baumann
Nov 20, 2015·Nature Structural & Molecular Biology·Linghe Xi, Thomas R Cech
Mar 4, 2020·Cancers·Nalini SrinivasRajiv Kumar
Nov 16, 2019·Cellular and Molecular Life Sciences : CMLS·Eric M SmithJayakrishnan Nandakumar
Nov 11, 2017·Wiley Interdisciplinary Reviews. RNA·Cherie MusgroveMichael D Stone

Related Concepts

Ter1 telomerase subunit, S pombe
DNA Helix Destabilizing Proteins
DNA, Fungal
Selfish DNA
RNA, Fungal
Schizosaccharomyces pombe
Structure-Activity Relationship

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

Genes & Development
Jiunn-Liang Chen, Carol W Greider
Nature Structural & Molecular Biology
Feng Qiao, Thomas R Cech
Proceedings of the National Academy of Sciences of the United States of America
Michael C Miller, Kathleen Collins
© 2021 Meta ULC. All rights reserved